School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China.
School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
Talanta. 2018 Feb 1;178:299-307. doi: 10.1016/j.talanta.2017.09.058. Epub 2017 Sep 20.
In present study, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared for specific recognition and selective enrichment of phloridzin from the leaves of Malus doumeri (Bois) A. Chev and rats' plasma. The magnetic FeO were prepared by the solvothermal reaction method and followed by the modification of TEOS and functionalization with APTES. Using functionalized FeO particles as the magnetic cores, phloridzin as template, ethylene glycol dimethacrylate (EGDMA) as cross-linker and 2,2-azobisisobutyonnitrile (AIBN) as initiator, the MMIPs were prepared through APTES to associate the template on the surface of the magnetic substrate. The structural features and morphological characterizations of MMIPs were performed by FT-IR, SEM, TEM, XRD, TGA and VSM. The adsorption experiments revealed that the MMIPs presented high selective recognition property to phloridzin. The selectivity experiment indicated that the adsorption capacity and selectivity of polymers to phloridzin was higher than that of baicalin and 2,3,5,4'-ttrahydroxy stilbene-2-O-β-D-glucoside. Furthermore, the MMIPs were employed as adsorbents for extraction and enrichment of phloridzin from the leaves of M. doumeri and rats' plasma. The recoveries of phloridzin in the leaves of M. doumeri ranged from 81.45% to 90.27%. The maximum concentration (C) of phloridzin in rats' plasma was detected as 12.19 ± 0.84μg/mL at about 15min after oral administration of phloridzin (200mg/kg). These results demonstrate that the prepared MMIPs are suitable for the selective adsorption of phloridzin from complex samples such as natural medical plants and biological samples.
在本研究中，成功制备了用于特异性识别和选择性富集梨皮素的磁性分子印迹聚合物（MMIPs），该聚合物来自杜梨（Bois）A. Chev 的叶子和大鼠血浆。通过溶剂热反应法制备了磁性 FeO，然后用 TEOS 进行改性，并通过 APTES 进行功能化。以功能化的 FeO 颗粒为磁性核，以根皮苷为模板，以乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，以 2,2-偶氮二异丁腈（AIBN）为引发剂，通过 APTES 将模板结合到磁性基质表面，制备 MMIPs。通过 FT-IR、SEM、TEM、XRD、TGA 和 VSM 对 MMIPs 的结构特征和形态特征进行了表征。吸附实验表明，MMIPs 对根皮苷具有高选择性识别性能。选择性实验表明，聚合物对根皮苷的吸附容量和选择性高于黄芩苷和 2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷。此外，MMIPs 被用作从杜梨叶和大鼠血浆中提取和富集根皮苷的吸附剂。杜梨叶中根皮苷的回收率在 81.45%至 90.27%之间。口服根皮苷（200mg/kg）后约 15min，大鼠血浆中根皮苷的最大浓度（C）检测为 12.19±0.84μg/mL。这些结果表明，所制备的 MMIPs 适用于从复杂样品（如天然药用植物和生物样品）中选择性吸附根皮苷。
