Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok, 10330, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 10120, Thailand.
Fish Shellfish Immunol. 2018 Jun;77:147-155. doi: 10.1016/j.fsi.2018.03.044. Epub 2018 Mar 28.
A two-domain Kazal-type serine proteinase inhibitor, SPIPm5, from Penaeus monodon was studied. Its transcript was expressed in all tissues tested including the hemocytes, stomach, gill, lymphoid organ, muscle, intestine and heart albeit less in hepatopancreas and eyestalk. The expression of SPIPm5 gene was also up-regulated by heat stress, white spot syndrome virus (WSSV) infection and yellow head virus (YHV) infection. Injection of recombinant rSPIPm5 protein into normal shrimp to mimic heat stress condition did not have or had little stimulating effect on the expression of other immune genes: crustinPm1, penaeidin3, penaeidin5, Hsp70, SPIPm2 and SPIPm5. Like some other proteinase inhibitors, the rSPIPm5 could inhibit the hemolymph proPO activity. In survival experiments, the rSPIPm5 could prolong the life of WSSV-infected shrimp similar to the effect of heat stress. The rSPIPm5 also helped the YHV-, Vibrio harveyi- and V. parahaemolyticus-infected shrimp survive longer. The increased endurance against microbial infection was due to the inhibitory effects presumably activated by rSPIPm5 on viral replication and bacterial growth but not the expression of antimicrobial peptides. Therefore, the SPIPm5 plays an important role in shrimp innate immunity against the viral and bacterial infection.
一种双结构域 Kazal 型丝氨酸蛋白酶抑制剂，来自斑节对虾的 SPIPm5，进行了研究。其转录本在所有测试组织中均有表达，包括血细胞、胃、鳃、淋巴器官、肌肉、肠道和心脏，但在肝胰腺和眼柄中表达较少。SPIPm5 基因的表达也被热应激、白斑综合征病毒 (WSSV) 感染和黄头病毒 (YHV) 感染上调。将重组 rSPIPm5 蛋白注射到正常虾中以模拟热应激条件，对其他免疫基因（如 crustinPm1、penaeidin3、penaeidin5、Hsp70、SPIPm2 和 SPIPm5）的表达没有或几乎没有刺激作用。像其他一些蛋白酶抑制剂一样，rSPIPm5 可以抑制血淋巴 proPO 活性。在生存实验中，rSPIPm5 可以延长 WSSV 感染虾的寿命，与热应激的效果相似。rSPIPm5 还帮助 YHV、哈维氏弧菌和副溶血弧菌感染的虾存活更长时间。对微生物感染的耐受力增加是由于 rSPIPm5 可能对病毒复制和细菌生长的抑制作用，而不是抗菌肽的表达所导致的。因此，SPIPm5 在虾先天免疫抵抗病毒和细菌感染中发挥重要作用。
