Visetnan Suwattana, Donpudsa Suchao, Supungul Premruethai, Tassanakajon Anchalee, Rimphanitchayakit Vichien
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
Fish Shellfish Immunol. 2014 Dec;41(2):526-30. doi: 10.1016/j.fsi.2014.09.036. Epub 2014 Oct 7.
A 5-domain Kazal type serine proteinase inhibitor SPIPm2 from Penaeus monodon is involved in innate immune defense against white spot syndrome virus (WSSV). To test which domains were involved, the 5 domains of SPIPm2 were over-expressed and tested against WSSV infection. By using hemocyte primary cell culture treated with each recombinant SPIPm2 domain along with WSSV, the expression of WSSV early genes ie1, WSV477 and late gene VP28 were substantially reduced as compared to other domains when the recombinant domain 2, rSPIPm2D2, was used. Injecting the WSSV along with rSPIPm2D2 but not with other domains caused delay in mortality rate of the infected shrimp. The results indicate that the SPIPm2D2 possesses strong antiviral activity and, hence, contributes predominantly to the antiviral activity of SPIPm2.
斑节对虾的一种5结构域卡扎尔型丝氨酸蛋白酶抑制剂SPIPm2参与对白斑综合征病毒（WSSV）的先天免疫防御。为了测试哪些结构域参与其中，对SPIPm2的5个结构域进行了过表达，并针对WSSV感染进行了测试。通过使用用每个重组SPIPm2结构域以及WSSV处理的血细胞原代细胞培养物，与其他结构域相比，当使用重组结构域2（rSPIPm2D2）时，WSSV早期基因ie1、WSV477和晚期基因VP28的表达显著降低。将WSSV与rSPIPm2D2一起注射，但不与其他结构域一起注射，导致受感染虾的死亡率延迟。结果表明，SPIPm2D2具有强大的抗病毒活性，因此，对SPIPm2的抗病毒活性起主要作用。
