Animal Physiology Laboratory, Department of Life Sciences, Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000 Sfax, Tunisia; Enzymes and Bioconversion Unit, National Engineering School of Sfax, University of Sfax, 3038 Sfax, Tunisia.
Animal Physiology Laboratory, Department of Life Sciences, Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000 Sfax, Tunisia.
Biomed Pharmacother. 2018 Jun;102:645-652. doi: 10.1016/j.biopha.2018.03.113. Epub 2018 Apr 5.
The present study pertains to the possible adverse effects of penconazole exposure on the lung of adult rats, and to the potential ability of vitamin E (Vit E) in mitigating the toxicity induced by this fungicide. Male Wistar rats were divided into four groups of six animals each: Group I (Controls): rats drank distilled water; Group II (PEN): rats received, by gavage, 50 mg/kg body weight (1/40 LD) of penconazole every 2 days during 10 days; Group III (Vit E): rats received daily 100 mg α-tocopherol acetate/kg body weight during 10 days by gavage; and Group IV (Vit E + PEN): rats received both vitamin E (100 mg α-tocopherol acetate/kg body weight) and penconazole (50 mg/kg body weight), being vitamin E given as a daily dosage and penconazole every 2 days, by gavage during 10 days. Results showed that penconazole induced oxidative stress in the lung demonstrated by an increase in malondialdehyde (+77%), hydrogen peroxide (+58%) and advanced oxidation protein product (+22%) levels, as compared to the controls. Furthermore, a decrease in the activities of catalase (-41%), superoxide dismutase (-45%), glutathione peroxidase (-23%) and acetylcholinesterase (-67%), and an increase in the levels of non-protein thiols (+17%), glutathione (+7%) and vitamin C (+44%) were registered. Abnormalities in lung histological sections such as alveolar edema, infiltration of inflammatory cells (leukocytes) and emphysema, were also observed following penconazole exposure. Vitamin E ameliorated the biochemical parameters, as well as the histological impairments induced by this fungicide. In conclusion, our study demonstrated that vitamin E, a natural antioxidant, was effective in alleviating penconazole-induced lung damage in Wistar rats.
本研究旨在探讨烯唑醇暴露对成年大鼠肺部的潜在不良影响，以及维生素 E（Vit E）减轻这种杀真菌剂毒性的潜在能力。雄性 Wistar 大鼠分为四组，每组 6 只：第 I 组（对照组）：大鼠饮用蒸馏水；第 II 组（PEN）：大鼠经灌胃给予 50mg/kg 体重（1/40 LD）烯唑醇，每 2 天 1 次，共 10 天；第 III 组（Vit E）：大鼠经灌胃给予每日 100mg α-生育酚乙酸酯/kg 体重，共 10 天；第 IV 组（Vit E+PEN）：大鼠同时给予维生素 E（100mg α-生育酚乙酸酯/kg 体重）和烯唑醇（50mg/kg 体重），维生素 E 作为每日剂量给予，烯唑醇每 2 天 1 次，共 10 天。结果表明，与对照组相比，烯唑醇诱导大鼠肺部氧化应激，丙二醛（+77%）、过氧化氢（+58%）和高级氧化蛋白产物（+22%）水平升高。此外，还观察到过氧化氢酶（-41%）、超氧化物歧化酶（-45%）、谷胱甘肽过氧化物酶（-23%）和乙酰胆碱酯酶（-67%）活性降低，非蛋白巯基（+17%）、谷胱甘肽（+7%）和维生素 C（+44%）水平升高。暴露于烯唑醇后，肺组织学切片也出现异常，如肺泡水肿、炎症细胞（白细胞）浸润和肺气肿。维生素 E 改善了这种杀真菌剂引起的生化参数和组织学损伤。综上所述，本研究表明，天然抗氧化剂维生素 E 可有效减轻 Wistar 大鼠烯唑醇引起的肺损伤。
