Animal Physiology Laboratory, Faculty of Science, BP1171, 3000 Sfax, University of Sfax, Tunisia.
Biomed Environ Sci. 2012 Jun;25(3):340-51. doi: 10.3967/0895-3988.2012.03.013.
To determine the efficiency of selenium and/or vitamin E to alleviate lung oxidative damage induced by dimethoate, an organophosphorus compound.
Adult Wistar rats were exposed during 30 days either to dimethoate (0.2 g/L of drinking water), dimethoate+selenium (0.5 mg/kg of diet), dimethoate+vitamin E (100 mg/kg of diet), or dimethoate+selenium+vitamin E.
Exposure to dimethoate caused oxidative stress in lung evidenced by an increase of malondialdehyde, protein carbonyl groups and advanced oxidation protein products. An increase in glutathione peroxidase, superoxide dismutase, catalase and a decrease in acetylcholinesterase and butyrylcholinesterase activities, glutathione, non-protein thiols and vitamins C levels were observed. Histopathological changes in lung tissue were noted as emphysema, hemorrhages and hemosiderin deposits. Co-administration of selenium or vitamin E to the diet of dimethoate treated rats ameliorated the biochemical parameters as well as histological impairments. The joint effect of these elements was more powerful in antagonizing dimethoate-induced lung oxidative damage.
We concluded that selenium and vitamin E ameliorated the toxic effects of this pesticide in lung tissue suggesting their role as potential antioxidants.
确定硒和/或维生素 E 对乐果（一种有机磷化合物）引起的肺氧化损伤的缓解作用。
成年 Wistar 大鼠连续 30 天暴露于乐果（饮用水中 0.2 g/L）、乐果+硒（饮食中 0.5 mg/kg）、乐果+维生素 E（饮食中 100 mg/kg）或乐果+硒+维生素 E 中。
暴露于乐果会导致肺中的氧化应激，表现为丙二醛、蛋白质羰基和高级氧化蛋白产物增加。谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶活性增加，乙酰胆碱酯酶和丁酰胆碱酯酶、谷胱甘肽、非蛋白巯基和维生素 C 水平降低。肺组织的组织病理学变化表现为肺气肿、出血和含铁血黄素沉积。将硒或维生素 E 共同添加到乐果处理大鼠的饮食中可改善生化参数以及组织学损伤。这些元素的联合作用在拮抗乐果诱导的肺氧化损伤方面更为有效。
我们得出结论，硒和维生素 E 改善了该农药对肺组织的毒性作用，表明它们具有作为潜在抗氧化剂的作用。
