Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Cytokine. 2018 Aug;108:115-119. doi: 10.1016/j.cyto.2018.03.025. Epub 2018 Mar 27.
Deregulated immunity is one of the most important factors implicated in recurrent pregnancy loss (RPL). The possible role of interleukin-33 (IL-33) and forkhead/winged helix transcription factor (Foxp3) in RPL have not been fully investigated. We aimed to evaluate IL-33 rs1929992 and Foxp3 rs2232365 single nucleotide polymorphisms (SNPs) and their serum levels in Egyptian RPL females. Blood samples were collected from 142 RPL patients and 123 women as healthy controls. IL-33 rs1929992 SNP was determined by polymerase chain reaction restriction fragment length polymorphism and Foxp3 rs2232365 SNP was determined using allele specific polymerase chain reaction. The serum IL-33 and Foxp3 levels were measured by enzyme linked immunosorbent assay. Foxp3 rs2232365 SNP showed statistically significant association with RPL. The risk of RPL was significantly higher in women carrying Foxp3 G allele than those carrying A allele. Lower serum levels of Foxp3 and IL-33 were observed in RPL patients than controls (P < 0.001). Foxp3 serum levels were much lower in carriers of G allele than those carrying A allele in all studied groups. Foxp3 rs2232365 SNP could be considered as a risk factor for RPL. The lowered serum levels of IL-33 and Foxp3 in RPL patients suggested that they might have an important role in the pathogenesis of the disease. Therefore, we hypothesized that Foxp3 polymorphisms may be important in RPL pathogenesis.
免疫失调是复发性流产（RPL）的最重要因素之一。白细胞介素 33（IL-33）和叉头/翼状螺旋转录因子（Foxp3）在 RPL 中的可能作用尚未得到充分研究。我们旨在评估埃及 RPL 女性中的 IL-33 rs1929992 和 Foxp3 rs2232365 单核苷酸多态性（SNP）及其血清水平。采集了 142 名 RPL 患者和 123 名健康对照者的血液样本。通过聚合酶链反应限制性片段长度多态性测定 IL-33 rs1929992 SNP，通过等位基因特异性聚合酶链反应测定 Foxp3 rs2232365 SNP。通过酶联免疫吸附试验测量血清 IL-33 和 Foxp3 水平。Foxp3 rs2232365 SNP 与 RPL 具有统计学显著相关性。携带 Foxp3 G 等位基因的女性发生 RPL 的风险明显高于携带 A 等位基因的女性。与对照组相比，RPL 患者的 Foxp3 和 IL-33 血清水平明显降低（P<0.001）。在所有研究组中，携带 G 等位基因的患者的 Foxp3 血清水平均明显低于携带 A 等位基因的患者。Foxp3 rs2232365 SNP 可被视为 RPL 的危险因素。RPL 患者中 IL-33 和 Foxp3 的血清水平降低表明它们可能在疾病的发病机制中起重要作用。因此，我们假设 Foxp3 多态性可能在 RPL 的发病机制中起重要作用。
