豚鼠阴道感染单纯疱疹病毒 2 型后黏膜免疫反应的转录分析。
Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2.
机构信息
Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555.
Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Department of Pediatrics, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555; Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd, Galveston, Texas, USA 77555.
出版信息
Virology. 2018 May;518:349-357. doi: 10.1016/j.virol.2018.03.019. Epub 2018 Mar 28.
Genital herpes infection in guinea pigs closely models human infection but tools for immune characterization are limited. Immunity to HSV infection at the vaginal epithelial surface was characterized in guinea pigs using PCR-based array analysis of vaginal swab samples. IFNγ was one of the most significantly upregulated genes throughout the infection and over 40% of genes with significantly altered expression were linked to IFNγ based on INTERFEROME analysis. IFNγ transcripts and biologically active IFNγ at the genital mucosa were confirmed by RTPCR and IFNγ reporter cells. Gene ontology analysis revealed activation of many biological processes related to genital immunity shared by humans and mice demonstrating the similarities of the local immune response to primary genital HSV-2 infection in guinea pigs and other established models. This transcription-based array will be useful for dissection of immunity during reactivation from latency, an infection outcome that is not well recapitulated by other animal models.
豚鼠的生殖器疱疹感染模型与人感染非常相似，但用于免疫特征分析的工具却很有限。本研究通过对阴道拭子样本进行基于 PCR 的阵列分析，对豚鼠阴道上皮表面的 HSV 感染免疫进行了研究。IFNγ是整个感染过程中上调最显著的基因之一，超过 40%表达发生显著改变的基因与 INTERFEROME 分析基于 IFNγ相关联。通过 RT-PCR 和 IFNγ报告细胞证实了生殖器黏膜中的 IFNγ 转录本和生物活性 IFNγ。基因本体分析显示，与人类和小鼠的生殖道免疫相关的许多生物学过程被激活，这表明豚鼠和其他已建立的模型中初次生殖器 HSV-2 感染的局部免疫反应具有相似性。这种基于转录的阵列将有助于从潜伏中重新激活期间的免疫分析，而其他动物模型并不能很好地重现这种感染结果。
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