Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Oil and POPs Research Group, Korea Institute of Ocean Science and Technology, Geoje 53201, South Korea.
Aquat Toxicol. 2018 Jun;199:21-29. doi: 10.1016/j.aquatox.2018.03.024. Epub 2018 Mar 22.
To understand the adverse outcome in response to bisphenol A and its analogs bisphenol F and S (BPA, BPF, and BPS), we examined acute toxicity, life parameter, and defensome in the marine rotifer Brachionus koreanus. Among the bisphenol analogs, BPA showed the highest acute toxicity and then BPF and BPS, accordingly in the view of descending magnitude of toxicity. In life parameters including life span and reproduction, BPA, BPF, and BPS were found to cause adverse effect. Both intracellular ROS level and GST activity were significantly increased (P < 0.05) in response to each dosage of bisphenol analogs exposures. In response to bisphenol analogs, defensomes of phase I, II, and III detoxification mechanism demonstrated inverse relationship between the lipophilicity of bisphenol analogs and the expression patterns of defensomes. BPA and BPF were found to have significant modulation (P < 0.05) in the expression of cytochrome P450 (CYP) and GST genes. In phase III, BPS with comparatively lower lipophilicity demonstrated highly diversified expressional pattern, suggesting that BPS is likely caused less toxicity compared to BPA and BPF. In this study, via phase I, II, and III detoxification mechanism, bisphenol A and its analogs F and S demonstrated specific detoxification mechanism in rotifer.
为了了解双酚 A 及其类似物双酚 F 和 S（BPA、BPF 和 BPS）的不良后果，我们研究了海洋轮虫 Brachionus koreanus 的急性毒性、生命参数和防御系统。在这些双酚类似物中，BPA 的急性毒性最高，其次是 BPF 和 BPS，毒性依次降低。在包括寿命和繁殖在内的生命参数方面，BPA、BPF 和 BPS 都被发现具有不良影响。细胞内 ROS 水平和 GST 活性在每种双酚类似物暴露剂量下均显著升高（P<0.05）。针对双酚类似物，I、II 和 III 期解毒机制的防御系统表现出双酚类似物脂溶性与防御系统表达模式之间的反比关系。BPA 和 BPF 被发现对细胞色素 P450（CYP）和 GST 基因的表达有显著调节（P<0.05）。在 III 期，BPS 具有较低的脂溶性，表现出高度多样化的表达模式，表明与 BPA 和 BPF 相比，BPS 可能引起的毒性较小。在这项研究中，通过 I、II 和 III 期解毒机制，双酚 A 及其类似物 F 和 S 在轮虫中表现出特定的解毒机制。
