Department of Civil and Environmental Engineering, University of Michigan, USA.
Department of Earth and Environmental Sciences, University of Michigan, USA.
Water Res. 2018 Jul 1;138:241-249. doi: 10.1016/j.watres.2018.03.047. Epub 2018 Mar 17.
Increasingly, technologies that use sulfide as an electron donor are being considered for nitrogen removal; however, our understanding of how sulfide affects microbial communities in nitrifying treatment processes is limited. In this study, we used batch experiments to quantify sulfide inhibition of both ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) using activated sludge from two full-scale treatment plants with distinct treatment processes. The batch experiments showed that NOB were more vulnerable to sulfide inhibition than AOB, and that inhibition constants (K) for NOB were distinct between the two treatment plants, which also had distinct nitrite oxidizing microbial communities. A Nitrospira-rich, less diverse NOB community was inhibited more by sulfide than a more diverse community rich in Nitrotoga and Nitrobacter. Therefore, sulfide-induced nitritation may be more successful in less diverse, Nitrospira-rich communities. Additionally, sulfide significantly influenced the activity of non-nitrifying microbial community members, as measured by 16S rRNA cDNA sequencing. Overall, these results indicate that sulfide has a strong impact on both nitrification and the activity of the underlying microbial communities, and that the response is community-specific.
越来越多的利用硫化物作为电子供体的技术被用于脱氮；然而，我们对硫化物如何影响硝化处理过程中的微生物群落的理解是有限的。在这项研究中，我们使用批式实验，使用来自两个具有不同处理工艺的全规模处理厂的活性污泥，量化了硫化物对氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）的抑制作用。批式实验表明，NOB 比 AOB 更容易受到硫化物的抑制，并且两个处理厂的 NOB 抑制常数（K）不同，这两个处理厂的亚硝酸盐氧化微生物群落也不同。富含 Nitrospira 的、多样性较低的 NOB 群落比富含 Nitrotoga 和 Nitrobacter 的多样性较高的群落更容易受到硫化物的抑制。因此，在多样性较低、富含 Nitrospira 的群落中，硫化物诱导的亚硝化可能更成功。此外，硫化物对非硝化微生物群落成员的活性有显著影响，这可以通过 16S rRNA cDNA 测序来衡量。总的来说，这些结果表明，硫化物对硝化作用和潜在微生物群落的活性都有很强的影响，而且这种影响是特定于群落的。
