Morrison C J, Brummer E, Stevens D A
Department of Medicine, Santa Clara Valley Medical Center, San Jose, California 95128.
Cell Immunol. 1987 Nov;110(1):176-82. doi: 10.1016/0008-8749(87)90111-0.
Peripheral blood polymorphonuclear neutrophils (PMN) from mice immunized with Blastomyces dermatitidis and then stimulated locally (intraperitoneally, ip) with B. dermatitidis antigen had enhanced killing of B. dermatitidis in vitro (54.4 +/- 19.49 of inoculum) compared to nonimmune mice (32.7 +/- 8.7%; P less than 0.02), nonimmune mice given antigen ip (30.6 +/- 14.0%; P less than 0.05), or immune mice not given antigen ip (15.4 +/- 9.9%; P less than 0.01). Peripheral blood PMN from all four groups had marked killing ability against Candida albicans (91.8-99.3% of inoculum). That the killing of B. dermatitidis was due to PMNs was demonstrated by lack of killing by isolated peripheral blood mononuclear cells from all four groups. A local immune reaction can result in enhancement of PMN fungicidal activity, and this is reflected even in peripheral blood PMN. We hypothesize this is an important component of normal host defenses against fungal infection, and likely other microbial infections. Enhancement of PMN microbicidal function by the soluble mediators presumed to be responsible for the effects observed may be an approach to immunomodulating therapy or prophylaxis of infection.
用皮炎芽生菌免疫小鼠，然后用皮炎芽生菌抗原进行局部（腹腔内，ip）刺激，这些小鼠外周血多形核中性粒细胞（PMN）在体外对皮炎芽生菌的杀伤能力增强（接种物的54.4±19.49%），相比之下，未免疫小鼠（32.7±8.7%；P<0.02）、腹腔内给予抗原的未免疫小鼠（30.6±14.0%；P<0.05）或未腹腔内给予抗原的免疫小鼠（15.4±9.9%；P<0.01）。所有四组小鼠的外周血PMN对白色念珠菌均有显著杀伤能力（接种物的91.8 - 99.3%）。所有四组的分离外周血单个核细胞均无杀伤作用，这证明了对皮炎芽生菌的杀伤是由PMN引起的。局部免疫反应可导致PMN杀菌活性增强，这甚至在外周血PMN中也有体现。我们推测这是正常宿主防御真菌感染以及可能其他微生物感染的重要组成部分。推测负责所观察到的效应的可溶性介质增强PMN杀微生物功能可能是一种免疫调节治疗或预防感染的方法。
