Brummer E, McEwen J G, Stevens D A
Clin Exp Immunol. 1986 Dec;66(3):681-90.
A characteristic of inflammation is the influx of polymorphonuclear neutrophils (PMN) from peripheral blood to the inflammatory reaction. We report on metabolic alterations and alterations in fungicidal activity in PMN elicited intraperitoneally with different inflammatory agents. The fungicidal activity of murine peripheral blood PMN (PB-PMN) against phagocytosable Candida albicans and nonphagocytosable Blastomyces dermatitidis was compared to that of murine inflammatory PMN. PMN elicited with sodium caseinate exhibited enhanced killing of B. dermatitidis (93 +/- 3%) compared to PB-PMN (38 +/- 11.7%). In contrast, thioglycollate medium elicited PMN had significantly less ability to kill B. dermatitidis (3 +/- 5%) than PB-PMN. Incubation of caseinate PMN with thioglycollate medium for 1 h significantly reduced their ability to kill B. dermatitidis (95% vs 25%). This effect was not due to cytotoxicity of thioglycollate medium for PMN. Candidacidal activity of inflammatory PMN (caseinate or proteose peptone-elicited) was not significantly greater than that of peripheral blood PMN. However, inflammatory PMN had significantly greater candidacidal activity than thioglycollate-elicited PMN.
炎症的一个特征是多形核中性粒细胞（PMN）从外周血流入炎症反应部位。我们报告了用不同炎症介质腹腔内诱导产生的PMN的代谢改变和杀真菌活性改变。将小鼠外周血PMN（PB-PMN）对可吞噬的白色念珠菌和不可吞噬的皮炎芽生菌的杀真菌活性与小鼠炎症PMN的杀真菌活性进行了比较。与PB-PMN（38±11.7%）相比，酪蛋白酸钠诱导产生的PMN对皮炎芽生菌的杀伤作用增强（93±3%）。相反，硫乙醇酸盐培养基诱导产生的PMN杀伤皮炎芽生菌的能力明显低于PB-PMN（3±5%）。将酪蛋白酸钠诱导产生的PMN与硫乙醇酸盐培养基孵育1小时后，其杀伤皮炎芽生菌的能力显著降低（从95%降至25%）。这种效应不是由于硫乙醇酸盐培养基对PMN的细胞毒性所致。炎症PMN（酪蛋白酸钠或蛋白胨诱导产生）的杀念珠菌活性并不显著高于外周血PMN。然而，炎症PMN的杀念珠菌活性明显高于硫乙醇酸盐诱导产生的PMN。
