Wang D S, Liang Y Y, Hu S C, Jiang X P
Beijing Institute for Cancer Research, Beijing.
Zhonghua Zhong Liu Za Zhi. 1987 Jul;9(4):265-8.
Photodynamic effect of HPD plus light on human stomach cancer cells (MGC-803) in the mitotic phase and interphase was studied. The synchronized mitotic cells were selected from the monolayer of asynchronous growing cells by shaking the culture flask. It was found that the killing frequency of HPD photosensitization was markedly different in the mitotic phase and interphase of synchronous growing MGC-803 cells, but a very small difference was found in HPD alone or light alone groups. The results show that, for adhesive cells in the two phases, there was a marked difference in damage pattern and recovery rate of mitosis 36 hr, 60 hr and 108 hr after photoradiation. The tolerance of mitotic cells to HPD plus light was much greater than that of the interphase cells. In the interphase, a great many of nude nuclei were seen and in the mitotic phase, cytokinesis was arrested resulting in many binucleated or multinucleated cells.
研究了血卟啉衍生物（HPD）加光照对处于有丝分裂期和间期的人胃癌细胞（MGC-803）的光动力效应。通过摇晃培养瓶，从非同步生长的单层细胞中筛选出同步有丝分裂细胞。结果发现，同步生长的MGC-803细胞在有丝分裂期和间期，HPD光致敏的杀伤频率有明显差异，但单独使用HPD组或单独光照组差异很小。结果表明，对于这两个阶段的贴壁细胞，光辐射后36小时、60小时和108小时，有丝分裂的损伤模式和恢复率存在明显差异。有丝分裂细胞对HPD加光照的耐受性远大于间期细胞。在间期，可见大量裸核，在有丝分裂期，胞质分裂受阻，导致许多双核或多核细胞。
