Gilmore Brian L, Varano A Cameron, Dearnaley William, Liang Yanping, Marcinkowski Bridget C, Dukes Madeline J, Kelly Deborah F
Virginia Tech Carilion Research Institute, Roanoke, VA, USA.
Translational Biology, Medicine, and Health Graduate Program, Virginia Tech, Blacksburg, VA, USA.
Methods Mol Biol. 2018;1764:45-58. doi: 10.1007/978-1-4939-7759-8_3.
Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining monolayer purification with silicon nitride microchips to create a tunable approach for the EM community.
技术上的最新进展使单颗粒电子显微镜（EM）迅速发展成为研究蛋白质组装体的首选工具。新开发的材料和方法为传统的EM样品制备提供了可行的替代方案。改进的脂质单层纯化试剂提供了相当大的灵活性，而超薄氮化硅膜为蛋白质复合物的结构研究提供了卓越的成像特性。在此，我们描述了将单层纯化与氮化硅微芯片相结合的步骤，为EM领域创建一种可调谐的方法。
