Gilmore Brian L, Winton Carly E, Demmert Andrew C, Tanner Justin R, Bowman Sam, Karageorge Vasilea, Patel Kaya, Sheng Zhi, Kelly Deborah F
Virginia Tech Carilion Research Institute, 2 Riverside Circle, Roanoke, Virginia 24016, United States.
School of Biomedical Engineering and Science, Virginia Tech, Blacksburg, VA 24061, United States.
Sci Rep. 2015 Sep 23;5:14440. doi: 10.1038/srep14440.
We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.
我们展示了一种新的分子工具包,用于研究在人类疾病背景下天然形成的蛋白质组装体。该系统采用可调节的微芯片,这些微芯片可以用可切换的适配分子进行修饰,以选择感兴趣的目标蛋白质,并使用分子显微镜对其进行分析。通过实施我们新的简化微芯片方法,我们首次能够直接可视化来自患者癌细胞的BRCA1基因调控复合物。