Emery D L, MacHugh N D, Ellis J A
International Laboratory of Research on Animal Diseases, Nairobi, Kenya.
Immunology. 1987 Oct;62(2):177-83.
Following prescapular lymphadenectomy, the cellular composition of afferent (peripheral) lymph from eight out of 13 calves in which the afferent and efferent lymphatic ducts had anastomosed was examined using monoclonal antibodies (mAb) specific for bovine lymphoid populations. Afferent lymph leucocytes (ALL) consisted of around 50% T lymphocytes, of which 35% and 14% expressed BoT4 and BoT8, respectively, 20-25% B lymphocytes and 23% non-lymphoid cells. When populations of non-lymphoid cells were prepared from ALL by adherence to gelatin-plasma-coated flasks, 81% contained alpha-napthyl acetate esterase (ANAE) and 70% were phagocytic, whereas the non-adherent population contained around 5% of these cell types, the cells being largely non-phagocytic and negative for ANAE. Following purification of non-lymphoid cells on the fluorescence-activated cell sorter (FACS) using IL-A22 (mAb detecting macrophages and polymorphonuclear leucocytes, PMN), only 72% of the positive cells contained ANAE, 19% were phagocytic, and 84% expressed Fc1 receptors. While the ALL depleted of IL-A22+ cells could not proliferate in the presence of specific antigen in culture, the addition of purified monocytes from peripheral blood leucocytes (PBL) or IL-A22-positive ALL to macrophage-depleted PBL or IL-A22-negative ALL promoted proliferative responses to specific antigens. Similar populations of non-lymphoid cells stimulated mixed leucocyte reactions (MLR) in allogeneic combinations. When these non-lymphoid cells were pulsed with specific antigens prior to addition to autologous PBL or ALL in culture, normal or gamma-irradiated but not glutaraldehyde-fixed cells from PBL or ALL induced proliferation and immunoglobulin (Ig) synthesis in responder cells. Proliferation was depressed in cultures containing mAb detecting BoT4, MHC class II antigens and a mAb which detected non-lymphoid cells in PBL; Ig synthesis was inhibited only by anti-class II mAb. Monocyte-depleted PBL or IL-A22-negative ALL responded specifically to autologous antigen-presenting cells which had been pulsed with the original immunogen and not other antigens.
在进行肩胛前淋巴结切除术后，使用针对牛淋巴细胞群体的单克隆抗体（mAb）对13头犊牛中8头的传入（外周）淋巴的细胞组成进行了检查，这些犊牛的传入和传出淋巴管已进行了吻合。传入淋巴白细胞（ALL）约由50%的T淋巴细胞组成，其中分别有35%和14%表达BoT4和BoT8，20 - 25%为B淋巴细胞，23%为非淋巴细胞。当通过贴壁于明胶 - 血浆包被的培养瓶从ALL中制备非淋巴细胞群体时，81%含有α - 萘乙酸酯酶（ANAE）且70%具有吞噬作用，而不贴壁群体中这些细胞类型约占5%，这些细胞大多无吞噬作用且ANAE呈阴性。在荧光激活细胞分选仪（FACS）上使用IL - A22（检测巨噬细胞和多形核白细胞（PMN）的mAb）对非淋巴细胞进行纯化后，仅72%的阳性细胞含有ANAE，19%具有吞噬作用，84%表达Fc1受体。虽然去除IL - A22 +细胞的ALL在培养中存在特异性抗原时不能增殖，但添加来自外周血白细胞（PBL）的纯化单核细胞或IL - A22阳性的ALL到去除巨噬细胞的PBL或IL - A22阴性的ALL中可促进对特异性抗原的增殖反应。相似的非淋巴细胞群体在同种异体组合中刺激混合淋巴细胞反应（MLR）。当在培养中添加到自体PBL或ALL之前用特异性抗原刺激这些非淋巴细胞时，来自PBL或ALL的正常或经γ射线照射但非戊二醛固定的细胞可诱导应答细胞增殖和免疫球蛋白（Ig）合成。在含有检测BoT4、MHC II类抗原的mAb以及检测PBL中非淋巴细胞的mAb的培养物中，增殖受到抑制；Ig合成仅被抗II类mAb抑制。去除单核细胞的PBL或IL - A22阴性的ALL对用原始免疫原而非其他抗原刺激的自体抗原呈递细胞有特异性反应。
