Sansone Marcelo, Sansone Ana Cláudia Miranda Brito, Shiga Tania Misuzu, Nascimento João Roberto Oliveira do
Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil; University of São Paulo - NAPAN - Food and Nutrition Research Center, São Paulo, Brazil.
Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
Food Res Int. 2016 Sep;87:125-133. doi: 10.1016/j.foodres.2016.07.003. Epub 2016 Jul 7.
Some diet components, such as certain indigestible polysaccharides from edible plants, may interact with the gut-associated lymphoid tissue and improve the host immune response to pathogens. The non-starch polysaccharides (NSP) from bananas are non-digestible carbohydrates that resemble some immunomodulatory polysaccharides occurring in the cell wall of cereals. Based on this similarity, the effects of the water-soluble NSP from two banana cultivars (Nanicão and Thap Maeo) on the phagocytic activity, nitric oxide (NO) and cytokines produced by cultured macrophages were investigated. An investigation into the monosaccharide composition and the oligomers released by enzymatic hydrolysis of the ultra-filtered fraction above 50kDa and the heat-treated fraction of water-soluble NSP from both cultivars revealed they are mostly composed of mannan and galacturonans (homogalacturonan, xylogalacturonan and rhamnogalacturonan). The NSP tested were able to activate the macrophages, but the effects on the phagocytic activity and the release of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide production were dependent on the polysaccharide concentration and the cultivar origin. Despite some specific differences, the NSP from Nanicão and Thap Maeo banana fruits may be considered prospective food immunomodulators, which contribute to the promotion of a more responsive immune system.
一些饮食成分，如可食用植物中的某些难消化多糖，可能与肠道相关淋巴组织相互作用，并改善宿主对病原体的免疫反应。香蕉中的非淀粉多糖（NSP）是难消化的碳水化合物，类似于谷物细胞壁中存在的一些免疫调节多糖。基于这种相似性，研究了两个香蕉品种（纳米卡奥和塔普梅奥）的水溶性NSP对培养巨噬细胞的吞噬活性、一氧化氮（NO）和细胞因子产生的影响。对两个品种50kDa以上超滤级分和水溶性NSP热处理级分经酶水解释放的单糖组成和低聚物的研究表明，它们主要由甘露聚糖和半乳糖醛酸聚糖（同型半乳糖醛酸聚糖、木糖半乳糖醛酸聚糖和鼠李糖半乳糖醛酸聚糖）组成。所测试的NSP能够激活巨噬细胞，但对吞噬活性以及肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）释放和一氧化氮产生的影响取决于多糖浓度和品种来源。尽管存在一些特定差异，但纳米卡奥和塔普梅奥香蕉果实中的NSP可被视为有望成为食物免疫调节剂，有助于促进免疫系统更具反应性。
