Song Ya, Wen Peng, Hao Huili, Zhu Minqian, Sun Yuanming, Zou Yuxiao, Requena Teresa, Huang Riming, Wang Hong
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences; Key Laboratory of Functional Foods, Ministry of Agriculture; Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
Polymers (Basel). 2020 Mar 8;12(3):615. doi: 10.3390/polym12030615.
is a horticultural plant and vital traditional Chinese herbal medicine. In our previous study, the characterization and immuno-enhancing effect of fruits polysaccharide 1 (PFP1), a water-eluted hetero-mannan from wild fruits, were investigated. Herein, another three salt-eluted novel polysaccharides, namely PFP2, PFP3, and PFP4, were obtained and structurally characterized. The results showed that PFP2, PFP3, and PFP4 were three structurally similar hetero-galacturonans with different molecular weights of 6.11 × 10, 4.37 × 10 and 3.48 × 10 g/mol, respectively. All three of these hetero-galacturonans are mainly composed of galacturonic acid, galactose, arabinose (75.69%, 80.39%, and 74.30%, respectively), and other monosaccharides including mannose, fucose, glucose, ribose, xylose, and glucuronic acid (24.31%, 19.61, and 25.70%, respectively), although differences in their backbone structure exist. Additionally, immunomodulatory assay indicated that the three hetero-galacturonans possess the ability to promote the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in RAW264.7 macrophages in a concentration-dependent manner ( < 0.05). Especially, PFP3 displayed a stronger enhancing effect than PFP2 and PFP4 at the minimum effective concentration. Therefore, the results suggested that the obtained three salt-eluted hetero-galacturonans, especially PFP3, could be utilized as immunomodulatory effectivity ingredients in nutritional/pharmaceutical industries.
是一种园艺植物，也是重要的传统中药材。在我们之前的研究中，对从野生果实中水洗得到的杂甘露聚糖——果实多糖1（PFP1）的特性及免疫增强作用进行了研究。在此，又获得了另外三种盐洗得到的新型多糖，即PFP2、PFP3和PFP4，并对其进行了结构表征。结果表明，PFP2、PFP3和PFP4是三种结构相似的杂半乳糖醛酸聚糖，分子量分别为6.11×10、4.37×10和3.48×10 g/mol。这三种杂半乳糖醛酸聚糖均主要由半乳糖醛酸、半乳糖、阿拉伯糖（分别为75.69%、80.39%和74.30%）以及包括甘露糖、岩藻糖、葡萄糖、核糖、木糖和葡萄糖醛酸在内的其他单糖（分别为24.31%、19.61%和25.70%）组成，尽管它们的主链结构存在差异。此外，免疫调节试验表明，这三种杂半乳糖醛酸聚糖具有以浓度依赖方式促进RAW264.7巨噬细胞中一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）产生的能力（<0.05）。特别是，在最低有效浓度下，PFP3的增强作用比PFP2和PFP4更强。因此，结果表明所获得的三种盐洗杂半乳糖醛酸聚糖，尤其是PFP3，可作为营养/制药行业中具有免疫调节功效的成分。
