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pH调节、均质化和透析过滤对中等蛋白质含量的乳蛋白浓缩物(MPC70)的物理化学、复性、功能和流变学特性的影响

Effect of pH adjustment, homogenization and diafiltration on physicochemical, reconstitution, functional and rheological properties of medium protein milk protein concentrates (MPC70).

作者信息

Meena Ganga Sahay, Singh Ashish Kumar, Gupta Vijay Kumar, Borad Sanket, Arora Sumit, Tomar Sudhir Kumar

机构信息

1Dairy Technology Division, ICAR- National Dairy Research Institute, Karnal, 132001 India.

2Dairy Chemistry Division, ICAR- National Dairy Research Institute, Karnal, 132001 India.

出版信息

J Food Sci Technol. 2018 Apr;55(4):1376-1386. doi: 10.1007/s13197-018-3052-y. Epub 2018 Feb 20.

Abstract

Poor solubility is the major limiting factor in commercial applications of milk protein concentrates (MPC) powders. Retentate treatments such as pH adjustment using disodium phosphate (NaHPO), also responsible for calcium chelation with homogenization and; its diafiltration with 150 mM NaCl solution were hypothesized to improve the functional properties of treated MPC70 powders. These treatments significantly improved the solubility, heat stability, water binding, dispersibility, bulk density, flowability, buffer index, foaming and emulsifying capacity of treated powders over control. Rheological behaviour of reconstituted MPC solutions was best explained by Herschel Bulkley model. Compared to rough, large globular structures with dents in control; majorly intact, separate, smaller particles of smooth surface, without any aggregation were observed in SEM micrograph of treated powders. Applied treatments are easy, cost-effective and capable to improve functional properties of treated powders that could replace control MPC70 powder in various food applications where protein functionality is of prime importance.

摘要

溶解性差是乳蛋白浓缩物(MPC)粉末商业应用中的主要限制因素。诸如使用磷酸氢二钠(NaHPO)进行pH调节等截留物处理,也负责通过均质化进行钙螯合;并且假设其用150 mM NaCl溶液进行渗滤可改善经处理的MPC70粉末的功能特性。与对照相比,这些处理显著改善了经处理粉末的溶解性、热稳定性、水结合性、分散性、堆积密度、流动性、缓冲指数、发泡和乳化能力。重构MPC溶液的流变行为最好用赫谢尔-巴克利模型来解释。与对照中粗糙、有凹痕的大球状结构相比;在经处理粉末的扫描电子显微镜图像中观察到主要是完整、分离、表面光滑的较小颗粒,没有任何聚集。所应用的处理方法简单、具有成本效益,并且能够改善经处理粉末的功能特性,在蛋白质功能至关重要的各种食品应用中可以替代对照MPC70粉末。

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