Jain Mukesh, Islam Sohidul, Rahman A S M Zisanur, Akhtar Sharmin, Hasan Kazi Nadim, Ahsan Gias Uddin, Khaleque Abdul, Hossain Maqsud
1Department of Biochemistry and Microbiology, North South University, Dhaka, 1229 Bangladesh.
2Department of Public Health, North South University, Dhaka, 1229 Bangladesh.
Virusdisease. 2018 Mar;29(1):54-60. doi: 10.1007/s13337-018-0431-y. Epub 2018 Feb 1.
Influenza A virus subtype H3 is a threat to public health and it is important to understand the evolution of the viruses for the surveillance and the selection of vaccine strains. Comparative analysis of four Bangladeshi isolates with isolates circulating other parts of the world based on three candidate genes hemagglutinin (HA), neuraminidase (NA), matrix protein (MA) showed no evidence of significant distinct subclade of viruses circulating in the country over the period of study. Despite these findings, we found N161S substitution in all four H3N2 influenza stains resulting in the gain of NSS160-162 glycosylation site. All H3N2 Influenza subtypes in the study had amino acid substitution at position 31 on the M2 protein (Aspartic acid to Asparagine) which is known to be responsible for amantadine drug resistance.
甲型H3流感病毒对公众健康构成威胁，了解病毒的进化对于监测和疫苗株的选择很重要。基于血凝素（HA）、神经氨酸酶（NA）、基质蛋白（MA）这三个候选基因，对四个孟加拉国分离株与世界其他地区流行的分离株进行比较分析，结果表明，在研究期间，该国流行的病毒没有明显独特亚分支的迹象。尽管有这些发现，但我们在所有四个H3N2流感毒株中都发现了N161S替换，导致获得了NSS160 - 162糖基化位点。研究中的所有H3N2流感亚型在M2蛋白的第31位都有氨基酸替换（天冬氨酸变为天冬酰胺），已知这会导致对金刚烷胺耐药。
