1 Office of Regulatory Science, Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration, College Park, Maryland 20740; and.
2 New Orleans District Office, Office of Regulatory Affairs (ORA), U.S. Food and Drug Administration, Jackson, Mississippi 39269, USA.
J Food Prot. 2018 May;81(5):700-704. doi: 10.4315/0362-028X.JFP-17-421.
A problem often encountered in the detection and identification of undeclared tree nut food allergens is the lack of analytical methods. This problem is accentuated by the current trend, whereby the primary methods used to detect food allergens are antibody-based enzyme-linked immunosorbent assays (ELISAs) and the development of analyte-specific antibodies takes months. The recently developed xMAP food allergen detection assay (xMAP FADA) has the ability to generate multiantigen profiles with tree nuts, thereby providing a potential solution to this problem. The xMAP FADA includes 22 antibodies targeting peanut, soy, and nine tree nuts. The high number of antibodies to a diverse group of tree nuts and legumes and the propensity of tree nuts to cross-react have enabled the development of multiantigen profiling, whereby an analyte reacts with the various antibodies to generate a profile. Recently, a question arose regarding the possible presence of pecan dust at a manufacturer of pecan products that also stored fresh produce. The lack of suitable pecan ELISAs created an analytical challenge that was resolved using multiantigen profiling with the xMAP FADA. Pecan was detected on swab samples by using multiantigen profiling and confirmed by DNA analysis. The use of multiantigen profiling provided an analytical capability beyond what was possible with an analyte-specific analytical method.
在检测和识别未申报的树坚果食物过敏原时，经常会遇到一个问题，那就是缺乏分析方法。由于目前主要用于检测食物过敏原的方法是基于抗体的酶联免疫吸附测定法（ELISA），而开发分析物特异性抗体需要数月的时间，这使得这个问题更加严重。最近开发的 xMAP 食物过敏原检测分析（xMAP FADA）具有检测树坚果的多抗原分析能力，从而为解决这个问题提供了一种潜在的解决方案。xMAP FADA 包括针对花生、大豆和九种树坚果的 22 种抗体。针对多种树坚果和豆类的大量抗体，以及树坚果之间的交叉反应倾向，使得多抗原分析得以发展，即分析物与各种抗体反应生成一个图谱。最近，在一家生产山核桃产品的制造商中出现了一个问题，即可能存在山核桃灰尘，而该制造商还储存新鲜农产品。由于缺乏合适的山核桃 ELISA，因此使用 xMAP FADA 进行多抗原分析解决了这个分析挑战。通过多抗原分析在拭子样本中检测到山核桃，并通过 DNA 分析进行了确认。多抗原分析的使用提供了一种超越分析物特异性分析方法的分析能力。
