a Faculty of Science, Department of Chemistry , Hacettepe University , Ankara , Turkey.
b Nanotechnology and Nanomedicine Division, Institute of Science , Hacettepe University , Ankara , Turkey.
J Biomater Sci Polym Ed. 2018 Aug;29(11):1302-1318. doi: 10.1080/09205063.2018.1457417. Epub 2018 Apr 2.
The novel nanosensor based on Surface Plasmon Resonance (SPR) was developed for sensitive and selective detection of ciprofloxacin via molecularly imprinted nanoparticles (MIP/NPs). NPs were synthesized through miniemulsion polymerization technique with methacrylic acid as a functional monomer. FTIR, SEM, zetasizer and contact angle measurements were used for the characterization of MIP/NPs. After modification the SPR chip surface, the nanosensor was used for detection of ciprofloxacin in aqueous solution. According to selected concentration range, the correlation coefficient and limit of detections were obtained as 0.993 (R) and 3.21 and 7.1 ppb in ultrapure water and SWW, respectively. Association kinetic analysis, Scatchard, Freundlich, Langmuir and Freundlich-Langmuir isotherms were also performed on the data to investigate adsorption behaviour of ciprofloxacin on the surface of nanosensor. Tetracycline and enrofloxacin were used as competitor agents to examine the selectivity of nanosensor. Performance of the SPR nanosensor was also investigated by using synthetic wastewater (SWW) for detection of CPX. The reusability of nanosensor was investigated and good repeatability was obtained with 5.81% RSD. As a result, selective, simple and low-cost method to detect ciprofloxacin in aqueous solution was developed by combining MIP/NPs and SPR.
基于表面等离子体共振（SPR）的新型纳米传感器通过分子印迹纳米粒子（MIP/NPs）的方法灵敏且选择性地检测环丙沙星。通过细乳液聚合技术，以甲基丙烯酸作为功能单体合成 NPs。傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、纳米粒度和 Zeta 电位分析仪以及接触角测量仪用于 MIP/NPs 的特性描述。在 SPR 芯片表面修饰后，纳米传感器用于检测水溶液中的环丙沙星。根据选定的浓度范围，在超纯水中和污水中，相关系数和检测限分别为 0.993（R）和 3.21 和 7.1 ppb。还对数据进行了缔合动力学分析、Scatchard、Freundlich、Langmuir 和 Freundlich-Langmuir 等温线分析，以研究环丙沙星在纳米传感器表面的吸附行为。四环素和恩诺沙星被用作竞争剂，以检验纳米传感器的选择性。还通过使用合成废水（SWW）检测 CPX 来研究 SPR 纳米传感器的性能。研究了纳米传感器的可重复使用性，获得了 5.81% RSD 的良好重复性。结果，通过将 MIP/NPs 和 SPR 结合，开发了一种用于检测水溶液中环丙沙星的选择性、简单且低成本的方法。
