Sui Weiguo, Gan Qing, Liu Fuhua, Chen Huaizhou, Liu Junfu, Dai Yong
1 Department of Nephrology, Guilin No. 181 Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin Key Laboratory of Kidney Diseases Research, Guilin, China.
2 College of Life Science, Guangxi Normal University, Guilin, China.
Tumour Biol. 2018 Apr;40(4):1010428318766928. doi: 10.1177/1010428318766928.
Recent studies have shown that circular ribonucleic acids have differential expression in some diseases. This study compared the expression levels of five circular ribonucleic acids between patients of primary hepatic carcinoma following liver transplantation and healthy individuals for searching a new diagnostic biomarker about primary hepatic carcinoma. We chose differentially expressed targeted circular ribonucleic acids according to fold change ≥2.0 or ≤-2.0 between circular ribonucleic acids microarray of perioperative liver transplantation and normal controls. Then we used the Arraystar home-made micro-ribonucleic acid target prediction software based on TargetScan and miRanda to predict circular ribonucleic acid/micro-ribonucleic acid interactions. And we assess the expression levels of hsa_circ_100571, hsa_circ_400031, hsa_circ_102032, hsa_circ_103096, and hsa_circ_102347 in the peripheral blood of normal controls and liver transplantation patients before transplantation and on the first, third, and seventh days after transplantation by real-time quantitative polymerase chain reaction. We chose five circular ribonucleic acids, two of which have been correlated with micro-ribonucleic acid-related carcinoma recurrence after liver transplantation, hepatocellular carcinoma and analyzed their expression with 2 method. The expression level of hsa_circ_100571 and hsa_circ_400031 on day 1 after liver transplantation was higher than pre-transplantation (p < 0.01), and these levels showed a declining trend on post-transplantation. The expression level of hsa_circ_102032 and hsa_circ_103096 on day 1 after liver transplantation was lower than pre-transplantation (p < 0.01) and decreased on post-transplantation. There were the significantly different expressions between the post-transplantation day 7 and normal control (p < 0.01). The expression level of hsa_circ_102347 on day 1 after liver transplantation was lower than pre-transplantation (p < 0.01). This expression showed a declining trend on post-transplantation, and the postoperative day 7 level was similar to normal control (p > 0.05). Five types of circular ribonucleic acid-related micro-ribonucleic acids had varying degrees of upregulation and downregulation between perioperative transplantation of primary hepatic carcinoma patients and normal controls; the hsa_circ_102347 is most likely to have association with primary hepatic carcinoma.
近期研究表明，环状核糖核酸在某些疾病中存在差异表达。本研究比较了肝移植后原发性肝癌患者与健康个体之间5种环状核糖核酸的表达水平，以寻找一种新的原发性肝癌诊断生物标志物。我们根据围手术期肝移植环状核糖核酸微阵列与正常对照之间的变化倍数≥2.0或≤ -2.0来选择差异表达的靶向环状核糖核酸。然后我们使用基于TargetScan和miRanda的Arraystar自制微小核糖核酸靶标预测软件来预测环状核糖核酸/微小核糖核酸相互作用。并且我们通过实时定量聚合酶链反应评估正常对照以及肝移植患者移植前、移植后第1天、第3天和第7天外周血中hsa_circ_100571、hsa_circ_400031、hsa_circ_102032、hsa_circ_103096和hsa_circ_102347的表达水平。我们选择了5种环状核糖核酸，其中两种已与肝移植、肝细胞癌后微小核糖核酸相关的癌复发相关，并采用两种方法分析它们的表达。肝移植后第1天hsa_circ_100571和hsa_circ_400031的表达水平高于移植前（p < 0.01），且这些水平在移植后呈下降趋势。肝移植后第1天hsa_circ_102032和hsa_circ_103096的表达水平低于移植前（p < 0.01），且在移植后降低。移植后第7天与正常对照之间存在显著差异表达（p < 0.01）。肝移植后第1天hsa_circ_102347的表达水平低于移植前（p < 0.01）。这种表达在移植后呈下降趋势，且术后第7天的水平与正常对照相似（p > 0.05）。原发性肝癌患者围手术期移植与正常对照之间5种环状核糖核酸相关的微小核糖核酸有不同程度的上调和下调；hsa_circ_102347最有可能与原发性肝癌相关。
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