Hsa_circ_0001649：一种环状 RNA，有望成为肝细胞癌的新型生物标志物。

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma.

机构信息

Shanghai Medical College of Fudan University, Shanghai, China.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Cancer Biomark. 2016;16(1):161-9. doi: 10.3233/CBM-150552.

DOI:10.3233/CBM-150552

PMID:26600397

Abstract

BACKGROUND

It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have been reported in hepatocellular carcinoma (HCC).

OBJECTIVE

To estimate clinical values of a circular RNA, Hsa_circ_0001649, in HCC.

METHODS

Expression level of hsa_circ_0001649 was detected in HCC and paired adjacent liver tissues by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Differences in expression level of hsa_circ_0001649 were analyzed using the paired t-test. Tests were performed between clinical information and hsa_circ_0001649 expression level by analysis of variance (ANOVA) or welch t-test and a receiver operating characteristics (ROC) curve was established to estimate the value of hsa_circ_0001649 expression as a biomarker in HCC.

RESULTS

hsa_circ_0001649 expression was significantly downregulated in HCC tissues (p = 0.0014) based on an analysis of 89 paired samples of HCC and adjacent liver tissues and the area under the ROC curve (AUC) was 0.63. Furthermore, hsa_circ_0001649 expression was correlated with tumor size (p = 0.045) and the occurrence of tumor embolus (p = 0.017) in HCC.

CONCLUSIONS

We first found hsa_circ_0001649 was significantly downregulated in HCC. Our findings indicate hsa_circ_0001649 might serve as a novel potential biomarker for HCC and may function in tumorigenesis and metastasis of HCC.

摘要

背景

已有研究表明环状 RNA（circRNA）与人类癌症相关，但在肝细胞癌（HCC）中报道较少。

目的

评估环状 RNA，Hsa_circ_0001649 在 HCC 中的临床价值。

方法

实时定量逆转录-聚合酶链反应（qRT-PCR）检测 HCC 及配对的癌旁肝组织中 hsa_circ_0001649 的表达水平。采用配对 t 检验分析 hsa_circ_0001649 表达水平的差异。采用方差分析（ANOVA）或 Welch t 检验分析 hsa_circ_0001649 表达水平与临床资料的关系，并绘制受试者工作特征（ROC）曲线评估 hsa_circ_0001649 表达作为 HCC 标志物的价值。

结果

基于 89 对 HCC 及癌旁肝组织的分析，hsa_circ_0001649 在 HCC 组织中表达明显下调（p = 0.0014），ROC 曲线下面积（AUC）为 0.63。此外，hsa_circ_0001649 表达与 HCC 肿瘤大小（p = 0.045）和肿瘤栓子的发生（p = 0.017）相关。