山羊和大鼠青春期中长链非编码 RNA 控制的发育基因表达的 RNA-seq 分析。
RNA-seq analysis of lncRNA-controlled developmental gene expression during puberty in goat & rat.
机构信息
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China.
Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei, 230036, Anhui, China.
出版信息
BMC Genet. 2018 Apr 2;19(1):19. doi: 10.1186/s12863-018-0608-9.
BACKGROUND
Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little is known about the function of lncRNAs (long noncoding RNAs) in puberty. Therefore, RNA-seq analysis were performed between goats and rats to clarify the roles of lncRNAs and mRNAs in the onset of puberty.
RESULTS
In the present study, the length of lncRNAs, the length of the open reading frame and the exon count were compared between the two species. Furthermore, functional annotation analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis of lncRNAs target genes and differentially expressed mRNA demonstrated the significantly enriched terms, such as AMPK signaling pathway, oxytocin signaling pathway, insulin secretion as well as pheromone receptor activity, and some other signaling pathways which were involved in the regulation of female puberty. Moreover, our results of siRNA interference in vitro showed the candidate lncRNA XLOC_446331 may play a crucial role in regulating female puberty.
CONCLUSION
In conclusion, the RNA-seq analysis between goat and rat provide novel candidate regulators for genetic and molecular studies on female puberty.
背景
青春期是雌性动物发育的关键阶段，标志着生殖能力的开始。然而，长链非编码 RNA（lncRNA）在青春期中的作用知之甚少。因此，本研究通过对山羊和大鼠进行 RNA-seq 分析，以阐明 lncRNA 和 mRNA 在青春期启动中的作用。
结果
本研究比较了两种物种的 lncRNA 长度、开放阅读框长度和外显子计数。此外，对 lncRNA 靶基因和差异表达 mRNA 的京都基因与基因组百科全书（KEGG）和基因本体论（GO）功能注释分析表明， AMPK 信号通路、催产素信号通路、胰岛素分泌以及信息素受体活性等显著富集的术语，以及其他一些参与调节雌性青春期的信号通路。此外，我们在体外进行的 siRNA 干扰结果表明，候选 lncRNA XLOC_446331 可能在调节雌性青春期中发挥关键作用。
结论
综上所述，山羊和大鼠之间的 RNA-seq 分析为雌性青春期的遗传和分子研究提供了新的候选调控因子。
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