Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Genes (Basel). 2022 Mar 28;13(4):606. doi: 10.3390/genes13040606.
The thyroid gland is a vital endocrine organ involved in the reproduction of animals via the regulation of hormone synthesis and secretion. LncRNAs have been proven to play important roles in reproductive regulation; however, the associated mechanism in the thyroid gland has not been clarified. In this study, we investigated to identify photoperiod-induced lncRNAs and mRNAs in the thyroid gland in Sunite ewes by comparing the expression profiles of short photoperiod (SP) and long photoperiods (LP). A total of 41,088 lncRNAs were identified in the thyroid gland through RNA-Seq. Functional analysis of differentially expressed lncRNAs using the R package revealed that reproductive hormone- and photoperiod response-related pathways, including the prolactin signaling, cAMP signaling, and circadian rhythm pathways, were significantly enriched. An mRNA-lncRNA interaction analysis suggested that the lncRNA trans targets and , and the lncRNA trans targets , both of these might be involved in seasonal sheep breeding reproduction. Together, these results will provide resources for further studies on seasonal reproduction in sheep.
甲状腺是一种重要的内分泌器官，通过调节激素的合成和分泌来参与动物的繁殖。lncRNAs 已被证明在生殖调节中发挥重要作用；然而，其在甲状腺中的相关机制尚未阐明。在这项研究中，我们通过比较短光照（SP）和长光照（LP）下的表达谱，研究了鉴定出的光周期诱导的 Sunite 母羊甲状腺中的 lncRNAs 和 mRNAs。通过 RNA-Seq 在甲状腺中鉴定出了 41,088 个 lncRNAs。使用 R 包对差异表达的 lncRNAs 进行功能分析表明，生殖激素和光周期反应相关途径，包括催乳素信号、cAMP 信号和昼夜节律途径，显著富集。mRNA-lncRNA 相互作用分析表明，lncRNA 转靶 和 ，lncRNA 转靶 ，这两个可能都参与了季节性绵羊繁殖的繁殖。总之，这些结果将为进一步研究绵羊的季节性繁殖提供资源。
