一种用于分析食品中黄曲霉毒素、赭曲霉毒素A、玉米赤霉烯酮、T-2毒素和杂色曲霉素的定量薄层色谱法。
A quantitative thin layer chromatography method for the analysis of aflatoxins, ochratoxin A, zearalenone, T-2 toxin and sterigmatocystin in foodstuffs.
作者信息
Tapia M O
机构信息
Departamento de Producción Animal, INTA Balcarce, Buenos Aires, Argentina.
出版信息
Rev Argent Microbiol. 1985;17(4):183-6.
A simple multimycotoxin analytical method was devised for the assay of 8 mycotoxins in foodstuff samples. The procedure consisted in the extraction of the samples with acetonitrile-4% KCl with the addition of HCl, defating with hexane and final extraction with methylene chloride. The mycotoxins were separated by bidimensional thin layer chromatography. The recovery was higher than 80% for all the mycotoxins. The limits of detection were 1.2 micrograms aflatoxins, 5 micrograms ochratoxin A, 20 micrograms sterigmatocystin, 100 micrograms T-2 toxin and 100 micrograms zearalenone/kg.
设计了一种简单的多霉菌毒素分析方法，用于测定食品样品中的8种霉菌毒素。该方法包括用含4%氯化钾的乙腈并添加盐酸对样品进行萃取，用己烷脱脂，最后用二氯甲烷萃取。霉菌毒素通过二维薄层色谱法进行分离。所有霉菌毒素的回收率均高于80%。黄曲霉毒素的检测限为1.2微克/千克，赭曲霉毒素A为5微克/千克，柄曲霉素为20微克/千克，T-2毒素和玉米赤霉烯酮为100微克/千克。
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