Roberts B A, Patterson D S
J Assoc Off Anal Chem. 1975 Nov;58(6):1178-81.
A multimycotoxin thin layer chromatographic screening method is described which is applicable to most animal feedstuffs. Interference from nonspecific lipid, pigment, and other components of simple and mixed feeds is reduced to a minimum by using a membrane cleanup step. Aflatoxins B1, B2, G1, and G2, citrinin, diacetoxyscirpenol, ochratoxin A, patulin, penitrem A, sterigmatocystin, T-2 toxin, and zearalenone may be reliably detected. The sensitivity of the method is generally low for mixed feeds but even so aflatoxin B1 can be detected at a level of 3 ppb and ochratoxin A at 80 ppb. While the basic method is less sensitive for sterigmatocystin (330 ppb), patulin (600 ppb), zearalenone (1000 ppb), and the trichothecenes (1000-4000 ppb), it may be adapted so as to reduce the above detection limits when the presence of these toxins is suspected. Lower levels may be detected in extracts of simple feeds.
本文描述了一种多霉菌毒素薄层色谱筛选方法，该方法适用于大多数动物饲料。通过膜净化步骤，可将简单饲料和混合饲料中脂质、色素及其他非特异性成分的干扰降至最低。黄曲霉毒素B1、B2、G1和G2、桔霉素、二醋酸镳草镰刀菌烯醇、赭曲霉毒素A、展青霉素、青霉震颤素、柄曲霉素、T-2毒素和玉米赤霉烯酮均可被可靠检测。该方法对混合饲料的灵敏度通常较低，但即便如此，黄曲霉毒素B1的检测限为3 ppb，赭曲霉毒素A的检测限为80 ppb。虽然该基本方法对柄曲霉素（330 ppb）、展青霉素（600 ppb）、玉米赤霉烯酮（1000 ppb）和单端孢霉烯族毒素（1000 - 4000 ppb）的灵敏度较低，但当怀疑存在这些毒素时，可对方法进行调整以降低上述检测限。在简单饲料提取物中可检测到更低水平的毒素。
