用于染色质免疫沉淀（ChIP）的交联染色质制备

Preparation of Cross-Linked Chromatin for ChIP.

作者信息

Kim Tae Hoon, Dekker Job

出版信息

Cold Spring Harb Protoc. 2018 Apr 2;2018(4):2018/4/pdb.prot082602. doi: 10.1101/pdb.prot082602.

Abstract

After living cells have been treated with formaldehyde to lock proteins to their DNA substrates, the DNA-protein complexes must be extracted and fragmented as described here. For ChIP-chip, it is not essential that a majority of chromatin fragments are ∼500 bp; an average fragment size ranging 1-2 kb is sufficient. However, for ChIP-seq, the average chromatin fragment should be <500 bp.

摘要

在用甲醛处理活细胞以将蛋白质锁定在其DNA底物上之后，必须按此处所述提取并片段化DNA-蛋白质复合物。对于芯片染色质免疫沉淀分析（ChIP-chip），大多数染色质片段为~500 bp并非必需；1-2 kb的平均片段大小就足够了。然而，对于染色质免疫沉淀测序（ChIP-seq），平均染色质片段应小于500 bp。