染色质免疫沉淀

ChIP.

作者信息

Kim Tae Hoon, Dekker Job

出版信息

Cold Spring Harb Protoc. 2018 Apr 2;2018(4):2018/4/pdb.prot082610. doi: 10.1101/pdb.prot082610.

Abstract

ChIP is a powerful method for analyzing protein-DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are ∼10-fold higher than for a standard ChIP assay.

摘要

染色质免疫沉淀技术（ChIP）是一种用于分析体内发生的蛋白质 - DNA 相互作用的强大方法。基本方案包括对交联的核蛋白复合物进行免疫沉淀，以及分离和分析与感兴趣的蛋白质相关的 DNA。此处所示的抗体和染色质用量适用于ChIP芯片和ChIP测序，比标准ChIP分析的用量高约10倍。