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人血浆中心房利钠肽的放射免疫测定

Radio-immunoassay of atrial natriuretic peptide in human plasma.

作者信息

Storm T L, Thamsborg G, Sykulski R, Keller N, Thomsen J, Larsen J

机构信息

Department of Medicine, Sundby Hospital, Copenhagen, Denmark.

出版信息

Scand J Clin Lab Invest. 1987 Nov;47(7):745-8.

PMID:2961046
Abstract

A highly sensitive and accurate radio-immunoassay for measurement of atrial natriuretic peptide in human plasma has been established. All reagents are commercially available. The sensitivity of the method is 1 pmol/l. The 50% intercept of the standard curve was at 22 fmol/tube and inter- and intra-assay precision were 10.9 and 5.3%, respectively. Plasma immunoreactive atrial natriuretic peptide levels measured in healthy subjects, during pregnancy, and in congestive cardiac failure were within the limits reported in the literature.

摘要

已建立一种用于测量人血浆中心房利钠肽的高度灵敏且准确的放射免疫分析法。所有试剂均可从市场购得。该方法的灵敏度为1皮摩尔/升。标准曲线的50%截距为22飞摩尔/管,批间和批内精密度分别为10.9%和5.3%。在健康受试者、孕期以及充血性心力衰竭患者中测得的血浆免疫反应性心房利钠肽水平均在文献报道的范围内。

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