Iwamoto Y, Robey F A, Graf J, Sasaki M, Kleinman H K, Yamada Y, Martin G R
Laboratory of Developmental Biology and Anomalies, National Institute of Drug Research, Bethesda, MD 20892.
Science. 1987 Nov 20;238(4830):1132-4. doi: 10.1126/science.2961059.
The invasion of tumor cells through basement membranes is a critical step in the formation of metastases. The binding of the malignant cells to laminin in the basement membranes allows their attachment and activates their invasiveness. Recently a synthetic nonapeptide from the B1 chain sequence of laminin was identified as a major site for cell binding. A pentapeptide within the nonapeptide sequence was found to reduce the formation of lung colonies in mice injected with melanoma cells and also to inhibit the invasiveness of the cells in vitro.
肿瘤细胞穿过基底膜的侵袭是转移形成过程中的关键步骤。恶性细胞与基底膜中的层粘连蛋白结合，使其能够附着并激活其侵袭性。最近，一种来自层粘连蛋白B1链序列的合成九肽被确定为细胞结合的主要部位。在该九肽序列中发现的一种五肽，可减少注射黑色素瘤细胞的小鼠肺部集落的形成，并在体外抑制细胞的侵袭性。
