School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India.
Mikrochim Acta. 2018 Apr 2;185(4):245. doi: 10.1007/s00604-018-2776-8.
A nanoporous coordination polymer (NPCP) was prepared from palladium(II) chloride and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole and is shown to act as a peroxidase mimetic. It can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO which is formed on enzymatic oxidation of glucose by glucose oxidase. Based on these findings, a sensitive glucose test was worked out at 652 nm where the intensity if the greenish-blue product is related to the actual concentration of glucose. Figures of merit include (a) rather low Km value (30 μM) which evidences the strong binding affinity of the NPCP toward glucose, (b) a high v (8.5 M·s), (c) a 47 nM detection limit, (d) a lifetime of a month, (e) a wide working pH range (2-10), and (f) a 25-80 °C temperature range. The assay was applied to non-invasive determination of glucose assay in tear, saliva where the detection limits are found to be 61 and 91 nM, respectively. Graphical abstract DSchematic of the mechanism of the peroxidase like catalytic activity of AHMT-Pd NPCP that was applied in a selective colorimetric method for glucose detection based on TMB oxidation in the presence of enzymatically generated HO.
一种纳米多孔配位聚合物（NPCP）由氯化钯和 4-氨基-3-肼基-5-巯基-1,2,4-三唑制备，被证明具有过氧化物酶模拟物的作用。它可以催化葡萄糖氧化酶酶促氧化葡萄糖生成的 HO 氧化 3,3',5,5'-四甲基联苯胺（TMB）。基于这些发现，在 652nm 处开发了一种灵敏的葡萄糖测试，其中绿色-蓝色产物的强度与葡萄糖的实际浓度有关。优点包括：（a）相当低的 Km 值（30μM），这证明 NPCP 对葡萄糖具有很强的结合亲和力；（b）高 v（8.5M·s）；（c）47nM 的检测限；（d）一个月的寿命；（e）宽工作 pH 范围（2-10）；（f）25-80°C 的温度范围。该测定法应用于非侵入性测定泪液和唾液中的葡萄糖含量，检测限分别为 61 和 91nM。
