Department of Inorganic Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology(HUST), 1st Dai Co Viet Road, Hanoi, Vietnam.
Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205, Paris Cedex 13, France.
Mikrochim Acta. 2018 Apr 27;185(5):270. doi: 10.1007/s00604-018-2804-8.
A method is described for the synthesis of a nanocomposite containing FeOOH and N-doped carbon nanosheets. The nanocomposite was synthesized by a hydrothermal method using a FeO/chitosan nanocomposite as the precursor. The nanocomposite displays peroxidase-like activity and catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO. This results in the formation of a blue colored product with an absorption maximum at 652 nm in the UV-vis spectra. Based on these findings, colorimetric assays were worked out for both hydrogen peroxide and glucose. The HO assay works in the 5 to 19 μM concentration range, and the limit of detection is 5 nM. The glucose assay works in the 8 μM to 0.8 mM concentration range and has a 0.2 μM detection limit. The method was successfully applied to the determination of glucose in human urine. Graphical abstract Schematic of the hydrothermal synthesis of a FeOOH/N-doped carbon nanocomposite. It was used to replace peroxidase enzyme for the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in a visual colorimetric test for glucose in human urine.
一种含有 FeOOH 和 N 掺杂碳纳米片的纳米复合材料的合成方法。该纳米复合材料通过水热法，以 FeO/壳聚糖纳米复合材料为前驱体制备。该纳米复合材料显示过氧化物酶样活性，并催化 HO 氧化 3,3',5,5'-四甲基联苯胺（TMB）。这导致在紫外可见光谱中在 652nm 处形成具有最大吸收的蓝色产物。基于这些发现，制定了用于过氧化氢和葡萄糖的比色测定法。HO 测定法在 5 至 19μM 的浓度范围内有效，检测限为 5nM。葡萄糖测定法在 8μM 至 0.8mM 的浓度范围内有效，检测限为 0.2μM。该方法成功应用于人尿中葡萄糖的测定。
