Park Hwayong, Kim Kwang Dong
Korean Medicine Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea.
Division of Applied Science, PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.
Mol Biol Rep. 2018 Jun;45(3):373-377. doi: 10.1007/s11033-018-4171-2. Epub 2018 Apr 2.
Insampaedok-san (IPS) is one of the East Asian traditional medicines which have been prescribed for hundreds of years to treat common cold and headache. Although many herbs and prescriptions are known to have significant activities against diseases, only a limited number of reports and scientific evidences on their efficacies are available. To identify anticancer effect against colon cancer, traditional prescription IPS and its fermented IPS (FIPS) were examined by in vitro molecular biological analysis. IPS water extract was fermented, lyophilized, and examined by cytotoxicity, cell cycle, and western blot assays, using cancer cell lines. Resultantly, FIPS showed significant cytotoxicities inducing caspase dependent apoptosis and activation of caspase-3 (CASP3) and poly (ADP-ribose) polymerase (PARP) cleavage in colon cancer cell line SW620. These findings can be useful for development of anticancer functional food or complementary and alternative medicine, with remaining in-depth molecular functional studies.
茵陈蒿汤（IPS）是一种已被用于治疗感冒和头痛数百年的东亚传统药物。尽管已知许多草药和方剂对疾病有显著作用，但关于它们疗效的报告和科学证据却很有限。为了确定其对结肠癌的抗癌作用，通过体外分子生物学分析对传统方剂IPS及其发酵后的IPS（FIPS）进行了研究。将IPS水提取物进行发酵、冻干，并使用癌细胞系通过细胞毒性、细胞周期和蛋白质免疫印迹分析进行检测。结果显示，FIPS在结肠癌细胞系SW620中表现出显著的细胞毒性，可诱导半胱天冬酶依赖性凋亡，并激活半胱天冬酶-3（CASP3）和聚（ADP-核糖）聚合酶（PARP）的裂解。这些发现对于抗癌功能性食品或补充替代医学的开发可能有用，不过仍需进行深入的分子功能研究。
