Barnett J B, Barfield L, Walls R, Joyner R, Owens R, Soderberg L S
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.
Toxicol Lett. 1987 Dec;39(2-3):263-74. doi: 10.1016/0378-4274(87)90242-6.
BALB/c mice were exposed to 0.0, 0.5 and 5.0 mg/kg maternal body weight hexachlorobenzene (HCB) throughout gestation by daily per os dosing of the females. At 45 days of age selected immune functions of the offspring were assessed. The delayed-type hypersensitivity (DTH) response to oxazolone was severely depressed in animals exposed to either 0.5 or 5.0 mg/kg HCB, however, only those animals exposed to 5.0 mg/kg HCB showed a significant decrease in their mixed lymphocyte response (MLR) levels. The ability of isolated spleen cells to undergo a blastogenic response to concanavalin A (ConA), phytohemagglutinin (PHA) and lipopolysaccharide (LPS) showed no significant changes due to HCB exposure. Similarly, no significant difference in the induction of direct hemolytic plaque-forming cells was seen. A significant increase in the relative distribution of splenic T cells and a significant decrease in splenic B cells was measured in the offspring of HCB-treated females. These results suggest that HCB is capable of affecting the development or maturation of the immune response in mice, perhaps at the T cell level.
在整个妊娠期，通过对雌性BALB/c小鼠每日经口给药，使其暴露于0.0、0.5和5.0毫克/千克母体体重的六氯苯（HCB）环境中。在子代45日龄时，评估其选定的免疫功能。暴露于0.5或5.0毫克/千克HCB的动物对恶唑酮的迟发型超敏反应（DTH）严重受抑，然而，只有那些暴露于5.0毫克/千克HCB的动物其混合淋巴细胞反应（MLR）水平显著降低。分离的脾细胞对刀豆蛋白A（ConA）、植物血凝素（PHA）和脂多糖（LPS）发生增殖反应的能力，未因HCB暴露而出现显著变化。同样，在直接溶血空斑形成细胞的诱导方面未观察到显著差异。在经HCB处理的雌性小鼠的子代中，测得脾T细胞的相对分布显著增加，脾B细胞显著减少。这些结果表明，HCB可能在T细胞水平影响小鼠免疫反应的发育或成熟。
