使用 TaqMan 阵列卡筛查坦桑尼亚发热病例的病因。
Use of TaqMan Array Cards to Screen Outbreak Specimens for Causes of Febrile Illness in Tanzania.
机构信息
Field Epidemiology and Laboratory Training Program, Dar es Salaam, Tanzania.
Center for Disease Control and Prevention, Atlanta, Georgia and Dar es Salaam, Tanzania.
出版信息
Am J Trop Med Hyg. 2018 Jun;98(6):1640-1642. doi: 10.4269/ajtmh.18-0071. Epub 2018 Mar 29.
Abstract
We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015-2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks.
摘要
我们描述了一种定制设计的分子诊断 TaqMan 阵列卡（TAC）的部署，用于筛查 2015-2017 年坦桑尼亚急性发热疾病暴发中血液中的 31 种细菌、原生动物和病毒病因。在坦桑尼亚卫生部通报的暴发事件中，派遣了流行病学家收集标本，并在 2 天内将标本运送到中央国家实验室，用 TAC 进行检测。该算法简化了调查，诊断了伤寒暴发，并排除了数十种其他病因。这种方法在国内是可用的，并且可以纳入诊断暴发的算法中。
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