Experimental Biology Division, Department of Experimental Biology, Defence Institute of Physiology and Allied Science, Defence Research and Development Organization, Timarpur, New Delhi, India.
Defence Research Laboratory, Tezpur, Assam, India.
Exp Mol Med. 2017 Dec 1;49(12):e404. doi: 10.1038/emm.2017.199.
This study investigates the therapeutic effect of a nanocurcumin formulation (NCF) containing nanocurcumin (NC) and pyrroloquinoline quinone (PQQ) on ameliorating hypoxia-induced stress in hypertrophied primary human ventricular cardiomyocytes (HVCM) under hypoxic conditions, as validated in a Sprague-Dawley rat model of chronic hypobaric hypoxia (cHH)-induced right ventricular hypertrophy (RVH). Based on our previous findings, here, we analyzed the improvement in the protective efficacy of NCF against mitochondrial damage. The electron transport chain Complexes' activities were analyzed as a chief operational center for mitochondrial homeostasis, along with key gene and protein markers for mitochondrial biogenesis, redox function, fatty acid oxidation, bio-energetic deficit and cell survival. NCF supplementation imparts cyto-protection from hypoxia-induced hypertrophy and damage in both in vitro and in vivo models while maintaining mitochondrial homeostasis better than NC and PQQ alone. This study proposes the use of NCF as a potential candidate molecule for imparting protection from high altitude-induced maladies in ascendants.
本研究探讨了一种含有纳米姜黄素（NC）和吡咯喹啉醌（PQQ）的纳米姜黄素制剂（NCF）在改善缺氧条件下肥大原代人心室肌细胞（HVCM）缺氧诱导应激中的治疗效果，该研究在慢性低压缺氧（cHH）诱导的右心室肥厚（RVH）的 Sprague-Dawley 大鼠模型中得到了验证。基于我们之前的研究结果，我们分析了 NCF 对线粒体损伤的保护效果的改善。电子传递链复合物的活性被分析为线粒体动态平衡的主要操作中心，同时还分析了线粒体生物发生、氧化还原功能、脂肪酸氧化、生物能量缺陷和细胞存活的关键基因和蛋白标志物。NCF 补充不仅在体外和体内模型中对缺氧诱导的肥大和损伤提供了细胞保护，而且比 NC 和 PQQ 单独使用更好地维持了线粒体动态平衡。本研究提出将 NCF 用作赋予高原相关疾病预防的潜在候选分子。
