[Allergologic-immunochemical study of various tree pollens. I. Characterization of antigen and allergen components in birch, beech, alder, hazel and oak pollens].

In previous experiments it was found that birch, beech, alder, hazel and oak are pollens with importance in pathogenesis of early pollinosis in our region of Central Europe. Using crossed immuno-(CIE) and radioimmunoelectrophoresis (CRIE) as well as immunoblotting following separation of pollen proteins by means of isoelectric focusing (IEF) and sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) the antigen and allergen patterns were investigated using a serum pool and individual sera. 29 antigens of birch, 32 antigens of beech, 29 antigens of alder, 24 antigens of hazel and 31 antigens of oak pollens as well as the thermostable major allergen B of birch pollen, the allergen Ca of beech, the allergen A of alder, the allergen Co of hazel and the allergen Q of oak pollen could be identified. Molecular weights and isoelectric points of these allergens were determined.