Egger C, Focke M, Bircher A J, Scherer K, Mothes-Luksch N, Horak F, Valenta R
Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria.
Clin Exp Allergy. 2008 Oct;38(10):1688-96. doi: 10.1111/j.1365-2222.2008.03092.x. Epub 2008 Aug 25.
Beech and oak pollen are potential allergen sources with a world-wide distribution.
We aimed to characterize the allergen profile of beech and oak pollen and to study cross-reactivities with birch and grass pollen allergens.
Sera from tree pollen-allergic patients with evidence for beech and oak pollen sensitization from Basel, Switzerland, (n=23) and sera from birch pollen-allergic patients from Vienna, Austria, (n=26) were compared in immunoblot experiments for IgE reactivity to birch (Betula pendula syn. verrucosa), beech (Fagus sylvatica) and oak (Quercus alba) pollen allergens. Subsequently, beech and oak pollen allergens were characterized by IgE inhibition experiments with purified recombinant and natural allergens and with allergen-specific antibody probes. Birch-, beech- and oak pollen-specific IgE levels were determined by ELISA.
Beech and oak pollen contain allergens that cross-react with the birch pollen allergens Bet v 1, Bet v 2 and Bet v 4 and with the berberine bridge enzyme-like allergen Phl p 4 from timothy grass pollen. Sera from Swiss and Austrian patients exhibited similar IgE reactivity profiles to birch, beech and oak pollen extracts. IgE levels to beech and oak pollen allergens were lower than those to birch pollen allergens.
IgE reactivity to beech pollen is mainly due to cross-reactivity with birch pollen allergens, and a Phl p 4-like molecule represented another predominant IgE-reactive structure in oak pollen. The characterization of beech and oak pollen allergens and their cross-reactivity is important for the diagnosis and treatment of beech and oak pollen allergy.
山毛榉和橡树花粉是分布于全球的潜在过敏原来源。
我们旨在描述山毛榉和橡树花粉的过敏原谱，并研究其与桦树和草花粉过敏原的交叉反应性。
比较来自瑞士巴塞尔的对山毛榉和橡树花粉致敏的树花粉过敏患者的血清（n = 23）和来自奥地利维也纳的桦树花粉过敏患者的血清（n = 26），通过免疫印迹实验检测其对桦树（垂枝桦 同物疣枝桦）、山毛榉（欧洲山毛榉）和橡树（白栎）花粉过敏原的IgE反应性。随后，通过用纯化的重组过敏原、天然过敏原和过敏原特异性抗体探针进行IgE抑制实验来鉴定山毛榉和橡树花粉过敏原。通过ELISA测定桦树、山毛榉和橡树花粉特异性IgE水平。
山毛榉和橡树花粉含有与桦树花粉过敏原Bet v 1、Bet v 2和Bet v 4以及来自梯牧草花粉的小檗碱桥酶样过敏原Phl p 4交叉反应的过敏原。瑞士和奥地利患者的血清对桦树、山毛榉和橡树花粉提取物表现出相似的IgE反应性谱。对山毛榉和橡树花粉过敏原的IgE水平低于对桦树花粉过敏原的IgE水平。
对山毛榉花粉的IgE反应性主要归因于与桦树花粉过敏原的交叉反应，并且一个Phl p 4样分子是橡树花粉中另一种主要的IgE反应性结构。山毛榉和橡树花粉过敏原的鉴定及其交叉反应性对于山毛榉和橡树花粉过敏的诊断和治疗很重要。
