采用新型酶联免疫吸附测定法评估先天性凝血因子 VII 缺乏症患者的凝血因子 VII 抗原
Evaluation of factor VII antigen in factor VII congenital deficiencies with a new ELISA assay.
作者信息
Tirindelli M C, Mariani G, Mazzucconi M G, Iacopino G, Carbonaro M, Ghirardini A, Motta M, Mandelli F
机构信息
Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Italia.
出版信息
Am J Hematol. 1987 Dec;26(4):313-21. doi: 10.1002/ajh.2830260404.
An evaluation of a new Enzyme Linked Immunosorbent Assay (ELISA) factor VII:Ag assay in factor VII congenital deficiencies was carried out. This assay was compared to factor VII:C assay and the Inhibitor Neutralization Assay (INA) for factor VII:Ag, both in normals and F VII-deficient patients. The correlations between ELISA F VII:Ag and VII:C, as well as the one between INA F VII:Ag and VII:C, were good (r = .86 and .81, respectively). The correlation between the two immunologic methods of assay in normals was fairly good (r = 0.73, P less than 0.001); whereas in mild F VII deficiencies (heterozygotes for F VII deficiencies), the two methods correlated very well (r = .96). In the severe deficiencies, ELISA F VII:Ag assay allowed the evaluation of F VII protein levels below 1 u/dl. The sensitivities of the INA and ELISA assays were evaluated by creating artificially prepared plasmas containing serial amounts of purified F VII: INA was unable to pick up F VII protein levels lower than 75 ng/ml, whereas the ELISA assay could detect up to 5 ng of F VII.
对一种用于评估先天性因子 VII 缺乏症的新型酶联免疫吸附测定（ELISA）因子 VII:Ag 检测方法进行了评估。将该检测方法与因子 VII:C 检测以及因子 VII:Ag 的抑制剂中和测定（INA）进行比较，比较对象包括正常人和因子 VII 缺乏症患者。ELISA 法检测的因子 VII:Ag 与 VII:C 之间的相关性良好（r 分别为 0.86 和 0.81），INA 法检测的因子 VII:Ag 与 VII:C 之间的相关性也良好（r 分别为 0.86 和 0.81）。正常人群中两种免疫检测方法之间的相关性相当好（r = 0.73，P 小于 0.001）；而在轻度因子 VII 缺乏症（因子 VII 缺乏症杂合子）中，两种方法的相关性非常好（r = 0.96）。在严重缺乏症中，ELISA 法检测因子 VII:Ag 能够评估低于 1 u/dl 的因子 VII 蛋白水平。通过制备含有系列纯化因子 VII 量的人工血浆来评估 INA 和 ELISA 检测方法的灵敏度：INA 无法检测到低于 75 ng/ml 的因子 VII 蛋白水平，而 ELISA 检测方法能够检测低至 5 ng 的因子 VII。
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