Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Bunkyo-ku, Tokyo 113-8657 , Japan.
Misaki Marine Biological Station (MMBS), Graduate School of Science and Center for Marine Biology , The University of Tokyo , Misaki, Miura , Kanagawa 238-0225 , Japan.
J Nat Prod. 2018 Apr 27;81(4):1108-1112. doi: 10.1021/acs.jnatprod.8b00101. Epub 2018 Apr 3.
Morphology-guided cell-based screening of the extract of a Mycale sp. marine sponge led to the isolation of two trisoxazole macrolides, miuramides A (1) and B (2), which induced characteristic morphological changes in 3Y1 cells. The structure of 1 including absolute configuration was elucidated by a combination of the analysis of spectroscopic data, derivatization, and degradation. Both compounds exhibit potent cytotoxicity against 3Y1 cells.
形态指导的基于细胞的海洋海绵 Mycale sp. 提取物筛选导致了两种三唑并大环内酯类化合物 miuramides A（1）和 B（2）的分离，它们在 3Y1 细胞中诱导了特征性的形态变化。通过分析光谱数据、衍生化和降解的组合，阐明了 1 的结构，包括绝对构型。这两种化合物均对 3Y1 细胞表现出很强的细胞毒性。
