Department of Medicine, Postgraduate Program in Health Sciences, Federal University of Sergipe, Rua Cláudio Batista, s/n, Cidade Nova, Aracaju, 49.100-000 Sergipe, Brazil.
Laboratory of Flavor and Chromatographic Analysis, Federal University of Sergipe, São Cristóvão, 49.100-000 Sergipe, Brazil.
Food Chem Toxicol. 2018 Jun;116(Pt B):86-99. doi: 10.1016/j.fct.2018.03.050. Epub 2018 Apr 1.
Citrus sinensis (L.) Osbeck is extensively cultivated worldwide and one of the most consumed fruits in the world. We evaluated the therapeutic properties of the methanol extract from Citrus sinensis fruit peel (CSMe) in high-fat diet-fed streptozotocin-induced insulin-resistant diabetic rats. Body weight, food intake, and water consumption were analysed. Biochemical and molecular biologic indices, and the expression of insulin receptor-induced signalling molecules were assessed to identify possible mechanisms. In addition, we conducted histology of pancreatic and adipose tissues. UHPLC-MS/MS analysis showed the presence of 17 dietary phenolics at substantial concentrations. High-fat diet-fed streptozotocin-induced diabetic rats administered CSMe (50 and 100 mg/kg) had reduced fasting blood glucose (56.1% and 55.7%, respectively) and plasma insulin levels (22.9% and 32.7%, respectively) compared with untreated diabetic control rats. CSMe reversed the biochemical abnormalities in diabetic rats, showed cytoprotective activity, and increased the intensity of the positive immunoreactions for insulin in pancreatic islets. CSMe treatment increased the expression of PPARγ in the adipose tissue and signalling molecules GLUT4 and insulin receptor. Our data suggest that CSMe could optimize glucose uptake of adipose tissues through the insulin-dependent signalling cascade mechanism and it should be investigated in the management of individuals with type 2 diabetes mellitus.
甜橙（Citrus sinensis（L.）Osbeck）在世界范围内广泛种植，是世界上消费最多的水果之一。我们评估了甜橙果皮甲醇提取物（CSMe）在高脂肪饮食喂养的链脲佐菌素诱导的胰岛素抵抗性糖尿病大鼠中的治疗特性。分析了体重、食物摄入量和饮水量。评估了生化和分子生物学指标以及胰岛素受体诱导的信号分子的表达，以确定可能的机制。此外，我们还对胰腺和脂肪组织进行了组织学检查。UHPLC-MS/MS 分析表明，存在 17 种具有相当浓度的膳食酚类物质。与未治疗的糖尿病对照大鼠相比，给予 CSMe（50 和 100mg/kg）的高脂肪饮食喂养的链脲佐菌素诱导的糖尿病大鼠的空腹血糖（分别为 56.1%和 55.7%）和血浆胰岛素水平（分别为 22.9%和 32.7%）降低。CSMe 逆转了糖尿病大鼠的生化异常，表现出细胞保护活性，并增加了胰岛中胰岛素阳性免疫反应的强度。CSMe 治疗增加了脂肪组织中 PPARγ和信号分子 GLUT4 和胰岛素受体的表达。我们的数据表明，CSMe 可以通过胰岛素依赖的信号级联机制优化脂肪组织的葡萄糖摄取，应该在 2 型糖尿病患者的管理中进行研究。
