Yoshikawa M, Watanabe M, Hozumi N
Mount Sinai Hospital Research Institute, Toronto, Ontario, Canada.
Cell Immunol. 1987 Dec;110(2):431-5. doi: 10.1016/0008-8749(87)90135-3.
In this report we have studied the effect of protease inhibitors on B-cell-antigen processing. As a source of antigen-presenting B cells we have utilized transformants transfected with a vector carrying immunoglobulin (Ig) genes specific for the hapten trinitrophenyl (TNP). B-cell-specific (TNP-proteins) and nonspecific antigen-presentation activities were blocked to the same extent upon addition of inhibitors for protease and endosomal function. Interestingly, the effect of leupeptin, a thiol protease inhibitor, varied depending on the antigen and helper T cells utilized. These results suggest that specific groups of proteases may be required for antigen processing so that discrete antigenic epitopes in association with major histocompatibility complex molecules can be recognized by interacting T cells.
在本报告中，我们研究了蛋白酶抑制剂对B细胞抗原加工的影响。作为抗原呈递B细胞的来源，我们利用了用携带对半抗原三硝基苯（TNP）具有特异性的免疫球蛋白（Ig）基因的载体转染的转化体。加入蛋白酶和内体功能抑制剂后，B细胞特异性（TNP蛋白）和非特异性抗原呈递活性受到同等程度的阻断。有趣的是，巯基蛋白酶抑制剂亮肽素的作用因所使用的抗原和辅助性T细胞而异。这些结果表明，抗原加工可能需要特定的蛋白酶组，以便相互作用的T细胞能够识别与主要组织相容性复合体分子相关的离散抗原表位。
