Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, 0454 Oslo, Norway.
National Institute of Aquatic Resources, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Viruses. 2018 Apr 2;10(4):170. doi: 10.3390/v10040170.
(PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout () with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus. Virus particles were purified by gradient ultracentrifugation and the complete coding sequences of PRV-3 were obtained by Illumina sequencing. When compared to PRV-1, the nucleotide identity of the coding regions was 80.1%, and the amino acid identities of the predicted PRV-3 proteins varied from 96.7% (λ1) to 79.1% (σ3). Phylogenetic analysis showed that PRV-3 belongs to a separate cluster. The region encoding σ3 were sequenced from PRV-3 isolates collected from rainbow trout in Europe. These sequences clustered together, but were distant from PRV-3 that was isolated from rainbow trout in Norway. Bioinformatic analyses of PRV-3 proteins revealed that predicted secondary structures and functional domains were conserved between PRV-3 and PRV-1. Rabbit antisera raised against purified virus or various recombinant virus proteins from PRV-1 all cross-reacted with PRV-3. Our findings indicate that despite different species preferences of the PRV subtypes, several genetic, antigenic, and structural properties are conserved between PRV-1 and-3.
(PRV-1) 可引起养殖大西洋鲑鱼的心脏和骨骼肌炎症 (HSMI)。最近，在具有 HSMI 样疾病的虹鳟鱼中发现了一种新型 PRV（以前称为 PRV-Om，现称为 PRV-3）。PRV 被认为是养殖鲑鱼中的一种新兴病原体。在这项研究中，对 PRV-3 进行了分子和抗原特性分析。红细胞是 PRV 的主要靶细胞，从实验性感染鱼采集的血液样本被用作病毒来源。病毒颗粒通过梯度超速离心进行纯化，并通过 Illumina 测序获得 PRV-3 的完整编码序列。与 PRV-1 相比，编码区的核苷酸同一性为 80.1%，预测的 PRV-3 蛋白的氨基酸同一性从 96.7%（λ1）到 79.1%（σ3）不等。系统进化分析表明，PRV-3 属于一个单独的聚类。从欧洲虹鳟鱼中分离到的 PRV-3 分离株中测序得到了编码 σ3 的区域。这些序列聚集在一起，但与从挪威虹鳟鱼中分离到的 PRV-3 相距甚远。对 PRV-3 蛋白的生物信息学分析表明，预测的二级结构和功能域在 PRV-3 和 PRV-1 之间是保守的。针对纯化病毒或 PRV-1 各种重组病毒蛋白制备的兔抗血清均与 PRV-3 发生交叉反应。我们的研究结果表明，尽管 PRV 亚型的物种偏好不同，但 PRV-1 和-3 之间存在一些遗传、抗原和结构特性是保守的。
