Duke O, Gordon Y, Panayi G S
Rheumatology Unit, United Medical School, Guy's Hospital, London.
Clin Exp Immunol. 1987 Oct;70(1):10-7.
We have tested the hypothesis that the spontaneous proliferation of synovial fluid mononuclear cells (SFMC) observed during in vitro culture is due to a mechanism analogous to the autologous mixed lymphocyte reaction (AMLR). Thus by observing the effect of addition of cyclosporin A, monoclonal anti-HLA-DR antibody, and recombinant interleukin 2 (rIL-2) on the spontaneous proliferation of SFMC from patients with rheumatoid arthritis (RA) and a wide range of seronegative spondyloarthritides (SN), we have demonstrated that this phenomenon is: (i) a T cell response, (ii) inhibited by the addition of cyclosporin A or monoclonal anti-HLA-DR antibody, (iii) enhanced by the addition of rIL-2 and (iv) the rIL-2 enhancement of the spontaneous proliferation is inhibited by a monoclonal anti-HLA-DR antibody. These observations suggest that the spontaneous proliferation of SFMC is at least in part an HLA-DR driven, IL-2 dependent event analogous to the AMLR, and support the concept derived from immunohistological studies of an important role for an antigen-presenting cell/T cell interaction occurring in the synovial membrane in inflammatory joint diseases such as RA.
体外培养过程中观察到的滑膜液单核细胞（SFMC）自发增殖是由于一种类似于自体混合淋巴细胞反应（AMLR）的机制。因此，通过观察添加环孢素A、单克隆抗HLA - DR抗体和重组白细胞介素2（rIL - 2）对类风湿关节炎（RA）患者及多种血清阴性脊柱关节炎（SN）患者SFMC自发增殖的影响，我们证明了这一现象：（i）是一种T细胞反应，（ii）添加环孢素A或单克隆抗HLA - DR抗体可抑制其增殖，（iii）添加rIL - 2可增强其增殖，且（iv）单克隆抗HLA - DR抗体可抑制rIL - 2对自发增殖的增强作用。这些观察结果表明，SFMC的自发增殖至少部分是由HLA - DR驱动、IL - 2依赖的事件，类似于AMLR，并支持了免疫组织学研究得出的概念，即在诸如RA等炎性关节疾病的滑膜中，抗原呈递细胞/T细胞相互作用发挥重要作用。
