Orta Olivia R, Tworoger Shelley S, Terry Kathryn L, Coull Brent A, Gelaye Bizu, Kirschbaum Clemens, Sanchez Sixto E, Williams Michelle A
a Harvard T.H. Chan School of Public Health , Harvard University , MA , USA.
b H. Lee Moffitt Cancer Center & Research Institute , Tampa , FL , USA.
Stress. 2018 Jul;21(4):355-365. doi: 10.1080/10253890.2018.1458088. Epub 2018 Apr 4.
Distal hair segments collected at delivery may allow for the assessment of maternal cortisol secretion in early pregnancy, an important time window for fetal development. Therefore, an investigation of the validity of distal hair cortisol concentrations is warranted. We examined the concordance between proximal and distal hair cortisol concentrations (HCC), both representing the first trimester of pregnancy. The study population was comprised of a random sample of 97 women participating in the Pregnancy Outcomes Maternal and Infant Study, a prospective cohort study of pregnant women attending prenatal clinics in Lima, Peru. Each participant provided two hair samples: once at enrollment [mean gestational age (GA) = 13.1 weeks] and again at full-term delivery (mean GA = 39.0 weeks). Hair segments reflecting the first trimester were: 3 cm hair segments closest to the scalp on the first hair sample (proximal) and 6-9 cm from the scalp on the second hair sample (distal). HCC was determined using Luminescence Immunoassay. A subset (N = 28) had both hair segments additionally analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). HCC values were log-transformed (logHCC), and proximal-distal differences tested using paired sample t-tests. Concordance was evaluated within and across assay types. LogHCC, measured using immunoassay, in distal hair segments was lower compared to proximal hair segments (1.35 versus 1.64 respectively; p = .02). No difference was observed using LC-MS/MS (1.99 versus 1.83, respectively; p=.33). Proximal-distal concordance was low within assay (immunoassay: Pearson = 0.27 and κ = 0.10; LC-MS/MS: Pearson = 0.37 and κ = 0.07). High correlation was observed across assays for both distal (Pearson = 0.78, p < .001; κ = 0.64) and proximal segments (Pearson = 0.96, p < .001; κ = 0.75). In conclusion, distal first-trimester hair segments collected at delivery have lower absolute HCC compared to HCC in proximal first trimester hair segments collected in early pregnancy, and are poorly concordant with HCC in proximal segments. Findings may inform the design of future studies.
分娩时采集的远端毛发段可能有助于评估孕早期母体皮质醇的分泌情况，而孕早期是胎儿发育的重要时间窗口。因此，有必要对远端毛发皮质醇浓度的有效性进行研究。我们检测了近端和远端毛发皮质醇浓度（HCC）之间的一致性，二者均代表妊娠的头三个月。研究人群包括97名参与母婴妊娠结局研究的女性随机样本，这是一项对秘鲁利马产前诊所孕妇进行的前瞻性队列研究。每位参与者提供了两份毛发样本：一份在入组时（平均孕周[GA]=13.1周），另一份在足月分娩时（平均GA=39.0周）。反映头三个月的毛发段分别为：第一份毛发样本中最靠近头皮的3厘米毛发段（近端）和第二份毛发样本中距离头皮6 - 9厘米的毛发段（远端）。使用发光免疫测定法测定HCC。一个子集（N = 28）的两份毛发段还使用液相色谱串联质谱法（LC - MS/MS）进行了额外分析。HCC值进行对数转换（logHCC），并使用配对样本t检验测试近端与远端的差异。在不同检测类型之间和之内评估一致性。使用免疫测定法测量的远端毛发段中的logHCC低于近端毛发段（分别为1.35和1.64；p = 0.02）。使用LC - MS/MS未观察到差异（分别为1.99和1.83；p = 0.33）。检测方法内部的近端与远端一致性较低（免疫测定法：Pearson = 0.27，κ = 0.10；LC - MS/MS：Pearson = 0.37，κ = 0.07）。对于远端（Pearson = 0.78，p < 0.001；κ = 0.64）和近端毛发段（Pearson = 0.96，p < 0.001；κ = 0.75），不同检测方法之间均观察到高度相关性。总之，与孕早期采集的近端头三个月毛发段中的HCC相比，分娩时采集的远端头三个月毛发段的绝对HCC较低，且与近端毛发段中的HCC一致性较差。研究结果可能为未来研究的设计提供参考。
