Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, 11451, Saudi Arabia.
Department of Obstetrics, Gynecology, King Khalid University Hospital & College of Medicine, King Saud University, Riyadh, Saudi Arabia.
BMC Complement Altern Med. 2018 Apr 3;18(1):121. doi: 10.1186/s12906-018-2176-y.
Cucumis sativus L. (cucumber), from the family Cucurbitaceae, is a therapeutic plant with various pharmacological benefits, broadly utilized as a part of complementary medicine (e.g., Unani, Ayurveda, Siddha, and Traditional Chinese). In light of past research discoveries, this plant had been chosen to consider its potential antibacterial action.
Extracts were purified by dialysis and ion exchange chromatography strategy and then assayed for antibacterial activity against four standard pathogenic bacterial strains known to cause foodborne infections and spoilage of food and herbal drugs. Antimicrobial peptides were extracted from seeds using a sodium phosphate citrate (pH 7.2) - CTAB cradle (pH 6.0).
The highest protein concentration was seen with elute fractions 1 and 3 (370 mg/mL) compared with elute fractions 2 and 4 (340 mg/mL). Among the bacteria utilized, E. coli was clearly the most sensitive out of selected four strains.
Our results suggest that Cucumis sativus L seeds extracts have significant potentials as new antimicrobial agents.
黄瓜（Cucumis sativus L.），葫芦科植物，具有多种药理学功效，是一种药用植物，在补充医学中广泛应用（例如，顺势疗法、阿育吠陀医学、悉达医学和传统中医）。鉴于过去的研究发现，选择这种植物来考虑其潜在的抗菌作用。
采用透析和离子交换层析策略对提取物进行纯化，然后对四种已知引起食源性感染和食物及草药腐败的标准致病菌进行抗菌活性检测。采用磷酸钠柠檬酸（pH 7.2）-CTAB 摇篮法（pH 6.0）从种子中提取抗菌肽。
与洗脱部分 2 和 4（340mg/mL）相比，洗脱部分 1 和 3（370mg/mL）的蛋白质浓度最高。在所选用的四种细菌中，大肠杆菌是最敏感的细菌。
我们的研究结果表明，黄瓜种子提取物具有作为新型抗菌剂的巨大潜力。
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