Wang H, Ng T B
Department of Microbiology, China Agricultural University, Beijing, China.
Biochem Biophys Res Commun. 2000 Dec 20;279(2):407-11. doi: 10.1006/bbrc.2000.3929.
A novel single-chained antifungal protein with a molecular weight of 13 kDa displaying an N-terminal sequence with marked similarity to embryo-abundant protein from the white spruce was isolated from the seeds of Ginkgo biloba using ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and then gel filtration on Superdex 75. The protein, designated ginkbilobin, exerted potent antifungal activity against a variety of fungi, including Botrytis cinerea, Mycosphaerella arachidicola, Fusarium oxysporum, Rhizoctonia solani, and Coprinus comatus. Ginkbilobin exhibited a moderate antibacterial action against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. It suppressed the activity of HIV-1 reverse transcriptase and the proliferation of murine splenocytes.
利用DEAE - 纤维素离子交换色谱、Affi - 凝胶蓝凝胶亲和色谱、SP - 琼脂糖离子交换色谱，然后通过Superdex 75凝胶过滤，从银杏种子中分离出一种分子量为13 kDa的新型单链抗真菌蛋白，该蛋白的N端序列与白云杉中富含胚胎的蛋白具有显著相似性。该蛋白被命名为银杏双黄酮，对多种真菌具有强大的抗真菌活性，包括灰葡萄孢、花生球腔菌、尖孢镰刀菌、立枯丝核菌和毛头鬼伞。银杏双黄酮对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌表现出中等抗菌作用。它抑制HIV - 1逆转录酶的活性和小鼠脾细胞的增殖。
