Lefebvre Fabio Alexis, Lécuyer Éric
Institut de Recherches Cliniques de Montréal (IRCM), Montréal, QC H2W 1R7, Canada.
Département de Biochimie, Université de Montréal, Montréal, QC H3T 1J4, Canada.
J Dev Biol. 2018 Mar 7;6(1):5. doi: 10.3390/jdb6010005.
Early development is punctuated by a series of pervasive and fast paced transitions. These events reshape a differentiated oocyte into a totipotent embryo and allow it to gradually mount a genetic program of its own, thereby framing a new organism. Specifically, developmental transitions that ensure the maternal to embryonic control of developmental events entail a deep remodeling of transcriptional and transcriptomic landscapes. provides an elegant and genetically tractable system to investigate these conserved changes at a dazzling developmental pace. Here, we review recent studies applying emerging technologies such as ribosome profiling, in situ Hi-C chromatin probing and live embryo RNA imaging to investigate the transcriptional dynamics at play during embryogenesis. In light of this new literature, we revisit the main models of zygotic genome activation (ZGA). We also review the contributions played by zygotic transcription in shaping embryogenesis and explore emerging concepts of processes such as transcriptional bursting and transcriptional memory.
早期发育以一系列普遍且快速的转变为特征。这些事件将一个已分化的卵母细胞重塑为一个全能胚胎，并使其能够逐渐启动自身的遗传程序，从而构建一个新的生物体。具体而言，确保发育事件从母体控制向胚胎控制转变的发育转变需要对转录和转录组景观进行深度重塑。 提供了一个优雅且遗传上易于处理的系统，以惊人的发育速度研究这些保守变化。在这里，我们回顾了最近的研究，这些研究应用了诸如核糖体谱分析、原位Hi-C染色质探测和活胚胎RNA成像等新兴技术，以研究胚胎发生过程中发挥作用的转录动态。鉴于这些新文献，我们重新审视了合子基因组激活（ZGA）的主要模型。我们还回顾了合子转录在塑造胚胎发生过程中的作用，并探讨了诸如转录爆发和转录记忆等过程的新兴概念。
