Department of Biology, University of Bergen, NO-5020, Bergen, Norway.
Faculty of Engineering and Science, Western Norway University of Applied Sciences, NO-6851, Sogndal, Norway.
FEMS Microbiol Ecol. 2018 May 1;94(5). doi: 10.1093/femsec/fiy059.
Methane (CH4) is one of the most abundant greenhouse gases in the atmosphere and identification of its sources and sinks is crucial for the reliability of climate model outputs. Although CH4 production and consumption rates have been reported from a broad spectrum of environments, data obtained from glacier forefields are restricted to a few locations. We report the activities of methanotrophic communities and their diversity along a chronosequence in front of a sub-Arctic glacier using high-throughput sequencing and gas flux measurements. CH4 oxidation rates were measured in the field throughout the growing season during three sampling times at eight different sampling points in combination with laboratory incubation experiments. The overall results showed that the methanotrophic community had similar trends of increased CH4 consumption and increased abundance as a function of soil development and time of year. Sequencing results revealed that the methanotrophic community was dominated by a few OTUs and that a short-term increase in CH4 concentration, as performed in the field measurements, altered slightly the relative abundance of the OTUs.
甲烷（CH4）是大气中含量最丰富的温室气体之一，其源汇的识别对于气候模型输出的可靠性至关重要。尽管已经从广泛的环境中报告了 CH4 的产生和消耗速率，但来自冰川前缘的资料仅限于少数几个地点。我们使用高通量测序和气体通量测量，报告了沿亚北极冰川前缘的一个时间序列中甲烷营养菌群落及其多样性的情况。在三个不同的采样时间，共在 8 个不同的采样点进行了野外测量，测量了整个生长季节的 CH4 氧化速率，并结合实验室培养实验进行了测量。总体结果表明，随着土壤发育和一年中时间的推移，甲烷营养菌群落的 CH4 消耗和丰度呈相似的增加趋势。测序结果表明，甲烷营养菌群落主要由少数几个 OTUs 组成，而在野外测量中进行的 CH4 浓度的短期增加，略微改变了 OTUs 的相对丰度。
